Callus induction in Portulaca oleracea L. by different hormone concentrations and explant types

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Heidargholinezhad Fereshteh; MORADI HOSSEIN; KARIMI MAHNAZ; AKBARPOUR VAHID

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Journal: NOVA BIOLOGICA REPERTA Year:2019 | Volume:6 | Issue:2 Page(s): 176-183

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Information Journal Paper

Title

Callus induction in Portulaca oleracea L. by different hormone concentrations and explant types

Author(s)

Heidargholinezhad Fereshteh | MORADI HOSSEIN | KARIMI MAHNAZ | AKBARPOUR VAHID | Issue Writer Certificate

Keywords

BAPQ1

medicinal plantQ1

purslaneQ1

tissue cultureQ2

2Q1

4-DQ1

Abstract

purslane (Portulaca oleracea L. ) contains valuable secondary metabolites such as Dopamin, Noradrenaline and Omega-3. This plant is used in various medicinal, food and hygienic industries as well as the treatment of different diseases such as diabetes, heart disease and pain relief. Callus induced from medicinal plants are used to increase the production of secondary metabolities in cell suspension culture and gene transfer. The purpose of this experiment was the study of different concentrations of BAP and 2, 4-D of two explants from leaf and shoot tips to produce callus. Leaf and shoot tip explants were used in MS with different concentrations of BAP at three levels (0, 1 and 2 mg/L) with 2, 4-D at three levels (0, 0. 5 and 1. 5 mg/L). Results showed that interactions between hormones and explants were significant in the percentage of callus induction, fresh weight and callus diameter at 1% level. The best result which was the leaf explant with 100% callus induction, 121 mg fresh weight and 5. 106 mm callus diameter was obtained by the combination of BAP 2 mg/L and 2, 4-D 0. 5 mg/L. Shoot tip explants with 75% callus induction, 106 mg fresh weight and 3. 03 mm diameter was obtained by the application of 1 mg/L BAP and 0. 5 mg/L 2, 4-D.

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APA: Copy

Heidargholinezhad, Fereshteh, MORADI, HOSSEIN, KARIMI, MAHNAZ, & AKBARPOUR, VAHID. (2019). Callus induction in Portulaca oleracea L. by different hormone concentrations and explant types. NOVA BIOLOGICA REPERTA, 6(2 ), 176-183. SID. https://sid.ir/paper/250488/en

Vancouver: Copy

Heidargholinezhad Fereshteh, MORADI HOSSEIN, KARIMI MAHNAZ, AKBARPOUR VAHID. Callus induction in Portulaca oleracea L. by different hormone concentrations and explant types. NOVA BIOLOGICA REPERTA[Internet]. 2019;6(2 ):176-183. Available from: https://sid.ir/paper/250488/en

IEEE: Copy

Fereshteh Heidargholinezhad, HOSSEIN MORADI, MAHNAZ KARIMI, and VAHID AKBARPOUR, “Callus induction in Portulaca oleracea L. by different hormone concentrations and explant types,” NOVA BIOLOGICA REPERTA, vol. 6, no. 2 , pp. 176–183, 2019, [Online]. Available: https://sid.ir/paper/250488/en

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