The study of impranil (DLN) polymer biodegradation by fungus Sarocladium kiliense

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Saffarzadeh Neshat; MOGHIMI HAMID

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Journal: NOVA BIOLOGICA REPERTA Year:2019 | Volume:6 | Issue:1 Page(s): 20-29

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Title

The study of impranil (DLN) polymer biodegradation by fungus Sarocladium kiliense

Author(s)

Saffarzadeh Neshat | MOGHIMI HAMID | Issue Writer Certificate

Keywords

bioremediationQ1

enzymatic activityQ1

plasticQ2

polyurethaneQ2

scanning electron microscopyQ2

Abstract

Impranil DLN is a class of plastics belonging to the polyurethane family with high application in textile industries. The aim of this study was to evaluate the potential of native strain to degrade impranil DLN. In this study, yeast strains were isolated from different areas and purified in minimal medium containing 1% impranil. Isolate NS-10 was selected as the superior strain capable of degrading impranil and identified through PCR and ITS gene. Esterase, urease and protease assays were carried out for the superior strain. Finally, the biodegradation of impranil was investigated. In total, 40 yeast strains were isolated and isolate NS-10 was selected as a superior strain based on impranil removal assay. NS-10 strain was identified as Sarocladium kiliense with 100% homology. Enzymatic assays showed that the S. kiliense could produce esterase, urease and protease. In addition, it could produce significant clear zones on impranil plates. Degradation rate for impranil was 100% for 10 g/l within 14 days. Finally, S. kiliense was taken to medium containing pure polyurethane film and the capacity of degradation was investigated by the scanning electron microscopy. Our results indicated that S. kiliense is capable of degrading impranil. These results could contribute to a better insight into the mechanism of plastic biodegradation.

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APA: Copy

Saffarzadeh, Neshat, & MOGHIMI, HAMID. (2019). The study of impranil (DLN) polymer biodegradation by fungus Sarocladium kiliense. NOVA BIOLOGICA REPERTA, 6(1 ), 20-29. SID. https://sid.ir/paper/250524/en

Vancouver: Copy

Saffarzadeh Neshat, MOGHIMI HAMID. The study of impranil (DLN) polymer biodegradation by fungus Sarocladium kiliense. NOVA BIOLOGICA REPERTA[Internet]. 2019;6(1 ):20-29. Available from: https://sid.ir/paper/250524/en

IEEE: Copy

Neshat Saffarzadeh, and HAMID MOGHIMI, “The study of impranil (DLN) polymer biodegradation by fungus Sarocladium kiliense,” NOVA BIOLOGICA REPERTA, vol. 6, no. 1 , pp. 20–29, 2019, [Online]. Available: https://sid.ir/paper/250524/en

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