Screening methods and enzyme profile of agricultural plant-endophytic bacteria

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Khosroshahi Samaneh; SALEHGHAMARI ENSIEH; AMOOZEGAR MOHAMMAD ALI; SAFFARIAN PARVANEH

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Journal: NOVA BIOLOGICA REPERTA Year:2020 | Volume:6 | Issue:4 Page(s): 415-423

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Title

Screening methods and enzyme profile of agricultural plant-endophytic bacteria

Author(s)

Khosroshahi Samaneh | SALEHGHAMARI ENSIEH | AMOOZEGAR MOHAMMAD ALI | SAFFARIAN PARVANEH | Issue Writer Certificate

Keywords

Bacillus halotoleransQ1

chitinaseQ1

hydrolytic enzymesQ2

proteaseQ1

xylanasQ2

Abstract

Nowadays plant endophytic bacteria have found diverse and useful applications in biotechnology; therefore, much attention has been paid to the isolation, identification, and evaluation of these microorganisms. Since the sterilizing plant tissue surfaces from epiphytic bacteria is difficulty, the efficacy of three different screening methods for endophytic bacteria including 1-HClO sterilization, 2-Periodic sterilization (modified tyndallization) and 3-Triton X100 and HClO sterilization, was evaluated in this study. The modified Tyndallization is an innovative method used in this study to appropriately remove the internal spores of epiphytic bacteria, considered to be an obstacle to the isolation of endophytes. Most of the endophytic bacteria were isolated from dicotyledons and leaves. Endophytic bacteria were also studied for the production of different hydrolase enzymes, whereas the protease enzyme was produced in a wide range of endophytic bacteria in greater quantities than other enzymes. The EndoA strain was molecularly identified and found to be 100% similar to Bacillus halotolerans.

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APA: Copy

Khosroshahi, Samaneh, SALEHGHAMARI, ENSIEH, AMOOZEGAR, MOHAMMAD ALI, & SAFFARIAN, PARVANEH. (2020). Screening methods and enzyme profile of agricultural plant-endophytic bacteria. NOVA BIOLOGICA REPERTA, 6(4 ), 415-423. SID. https://sid.ir/paper/250591/en

Vancouver: Copy

Khosroshahi Samaneh, SALEHGHAMARI ENSIEH, AMOOZEGAR MOHAMMAD ALI, SAFFARIAN PARVANEH. Screening methods and enzyme profile of agricultural plant-endophytic bacteria. NOVA BIOLOGICA REPERTA[Internet]. 2020;6(4 ):415-423. Available from: https://sid.ir/paper/250591/en

IEEE: Copy

Samaneh Khosroshahi, ENSIEH SALEHGHAMARI, MOHAMMAD ALI AMOOZEGAR, and PARVANEH SAFFARIAN, “Screening methods and enzyme profile of agricultural plant-endophytic bacteria,” NOVA BIOLOGICA REPERTA, vol. 6, no. 4 , pp. 415–423, 2020, [Online]. Available: https://sid.ir/paper/250591/en

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