ANTIFUNGAL EFFECT OF SILVER NANOPARTICLES BIOSYNTHESIZED BY SACCHAROMYCES CEREVISIAE ON CANDIDA ALBICANS

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OMIDI BEHIN; Tabatabaee Mitra Sadat; KASHANI KIMIA; OSTADI YAHYA

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Journal: JOURNAL OF APPLIED MICROBIOLOGY IN FOOD INDUSTRY Year:2016 | Volume:2 | Issue:4 Page(s): 30-44

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Title

ANTIFUNGAL EFFECT OF SILVER NANOPARTICLES BIOSYNTHESIZED BY SACCHAROMYCES CEREVISIAE ON CANDIDA ALBICANS

Author(s)

OMIDI BEHIN | Tabatabaee Mitra Sadat | KASHANI KIMIA | OSTADI YAHYA | Issue Writer Certificate

Keywords

SILVER NANOPARTICLESQ2

BIOSYNTHESISQ1

SACCHAROMYCES CEREVISIAEQ2

CANDIDA ALBICANQ2

Abstract

SILVER NANOPARTICLES have been synthesized by several methods, but each of them has disadvantages. Chemical methods, have high toxicity and low stability, and physical methods, are costly and low-efficient More recently, researchers have found a new technique for synthesizing AgNPs, using plants and microorganisms such as bacteria, fungi, and actinomycetes. This method is called "eco-friendly", "green synthesis" or "biosynthesis". The aim of this investigation was, BIOSYNTHESIS of AgNPs using SACCHAROMYCES CEREVISIAE, which is one of the safe microorganisms that the AgNPs synthesized by it, are suitable for using in pharmacology and medicine purposes.Saccharomyces cerevisiae was cultured in sabraud dextrose broth and incubated at 28-30°c for 24 h, then it was centrifuged, and its supernatant was added to the silver nitrate solution with the concentration of 0.001, 0.002 and 0.003 Mat pH: 5.6, 7 and 9, then incubated at 28 -35 and 37°c. The formation of AgNPs, was monitored by colorchanging, uv-vis spectroscopy, TEM, XRD and FTIR. Antifungal effect of AgNPs on CANDIDA ALBICANs investigated by disc diffusion method. MIC and MFC were also measured.The results, showed that SACCHAROMYCES CEREVISIAE have had the great potential for syothesizing of AgNPs in the size of 27 nm and have shown the antifungal effect in the concentration of 37±0.29 mg/ml.In this study, we have reported a simple, biological, easy and nontoxic method for synthesis of SILVER NANOPARTICLES, which have had suitable antifungal effect on CANDIDA ALBICANs, by using one of the nonpathogenic microorganisms.

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APA: Copy

OMIDI, BEHIN, Tabatabaee, Mitra Sadat, KASHANI, KIMIA, & OSTADI, YAHYA. (2016). ANTIFUNGAL EFFECT OF SILVER NANOPARTICLES BIOSYNTHESIZED BY SACCHAROMYCES CEREVISIAE ON CANDIDA ALBICANS. JOURNAL OF APPLIED MICROBIOLOGY IN FOOD INDUSTRY, 2(4), 30-44. SID. https://sid.ir/paper/254881/en

Vancouver: Copy

OMIDI BEHIN, Tabatabaee Mitra Sadat, KASHANI KIMIA, OSTADI YAHYA. ANTIFUNGAL EFFECT OF SILVER NANOPARTICLES BIOSYNTHESIZED BY SACCHAROMYCES CEREVISIAE ON CANDIDA ALBICANS. JOURNAL OF APPLIED MICROBIOLOGY IN FOOD INDUSTRY[Internet]. 2016;2(4):30-44. Available from: https://sid.ir/paper/254881/en

IEEE: Copy

BEHIN OMIDI, Mitra Sadat Tabatabaee, KIMIA KASHANI, and YAHYA OSTADI, “ANTIFUNGAL EFFECT OF SILVER NANOPARTICLES BIOSYNTHESIZED BY SACCHAROMYCES CEREVISIAE ON CANDIDA ALBICANS,” JOURNAL OF APPLIED MICROBIOLOGY IN FOOD INDUSTRY, vol. 2, no. 4, pp. 30–44, 2016, [Online]. Available: https://sid.ir/paper/254881/en

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