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Information Journal Paper

Title

OPTIMIZATION OF CULTURE MEDIA FOR MICROBIAL HYALURONIC ACID PRODUCTION IN A BATCH SYSTEM

Pages

  1-9

Abstract

 In the present study, the microbial production of HYALURONIC ACID by wild type STREPTOCOCCUS ZOOEPIDEMICUS in semi defined medium in shake flask and 5 liters fermenter has studied. At first the culture condition and medium composition optimized. To experimental design, L-9 array of taguchi approach is used. The effects of glucose, yeast extract, sulphate and phosphate salts concentration and medium pH on HYALURONIC ACID production in BATCH CULTURE in shakeflask was studied. Based on experimental data analysis, optimum condition for HYALURONIC ACID production was: 30 g/L glucose, 20 g/L yeast extract, 7.5 g/L sulphate and phosphate salts and medium pH 7. Of the culture condition examined, the yeast extract concentration had the most pronounced effect on the HYALURONIC ACID production. In optimum condition, HYALURONIC ACID production at 206 mg/L and 800 mg/L, in shake flask and 5 liters fermenter, in BATCH CULTURE was obtained.

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    APA: Copy

    AMIRSADEGHI, A.R., VASHEGHANI FARAAHANI, E., & SHAHHOSSEINI, SH.. (2009). OPTIMIZATION OF CULTURE MEDIA FOR MICROBIAL HYALURONIC ACID PRODUCTION IN A BATCH SYSTEM. NASHRIEH SHIMI VA MOHANDESI SHIMI IRAN (PERSIAN), 28(1), 1-9. SID. https://sid.ir/paper/26008/en

    Vancouver: Copy

    AMIRSADEGHI A.R., VASHEGHANI FARAAHANI E., SHAHHOSSEINI SH.. OPTIMIZATION OF CULTURE MEDIA FOR MICROBIAL HYALURONIC ACID PRODUCTION IN A BATCH SYSTEM. NASHRIEH SHIMI VA MOHANDESI SHIMI IRAN (PERSIAN)[Internet]. 2009;28(1):1-9. Available from: https://sid.ir/paper/26008/en

    IEEE: Copy

    A.R. AMIRSADEGHI, E. VASHEGHANI FARAAHANI, and SH. SHAHHOSSEINI, “OPTIMIZATION OF CULTURE MEDIA FOR MICROBIAL HYALURONIC ACID PRODUCTION IN A BATCH SYSTEM,” NASHRIEH SHIMI VA MOHANDESI SHIMI IRAN (PERSIAN), vol. 28, no. 1, pp. 1–9, 2009, [Online]. Available: https://sid.ir/paper/26008/en

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