INVESTIGATION OF EXPRESSION OF CYP19 (A) AND REPRODUCTION IN DANIO RERIOFED A DIET ENRICHED WITH VITEX AGNUS CASTUS EXTRACT

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ENAYAT GHOLAMPOUR TAYEBEH; JAFARI VALIOLAH; IMANPOUR MOHAMAD REZA; KOLANGI MIANDARE HAMED

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY Year:2016 | Volume:4 | Issue:3 Page(s): 27-42

Download Full-Text

Persian Verion

View:

772

Download:

Cites:

Information Journal Paper

Title

INVESTIGATION OF EXPRESSION OF CYP19 (A) AND REPRODUCTION IN DANIO RERIOFED A DIET ENRICHED WITH VITEX AGNUS CASTUS EXTRACT

Author(s)

ENAYAT GHOLAMPOUR TAYEBEH | JAFARI VALIOLAH | IMANPOUR MOHAMAD REZA | KOLANGI MIANDARE HAMED | Issue Writer Certificate

Keywords

REPRODUCTIONQ2

CYP19 (A)Q1

VITEX AGNUS CASTUSQ1

DANIO RERIOQ1

Abstract

P450 aromatase (CYP19 (a)) is the key steroidogenic enzyme responsible for conversion of androgens to estrogens which play a critical role in developmental and sex differentiation. Hence, this study was done to evaluate the effect of VITEX AGNUS CASTUS hydroalchoholic extract on reproductive performance, sex ratio and expression of CYP19 (a), one of the most important gene related to gender, using RT-PCR method in zebrafish. The larvae were fed post-hatching with diets containing extracts of four levels included 0 (control group T0), 5 (T1), 10 (T2) and 15 (T3) g/Kg food for 45 days. For gene expression studies, samples of larvae at 4, 7, 10, 15, 30 and 45 days post-hatching were collected. Expression of b-actin housekeeping genes was normalized as reference gene. Gene expression was evaluated by using 2-ΔΔCT method. The reproductive performance was significantly better in T2 and T3 than other treatments (P<0.05). With increasing concentration of extract in the diet, the ratio of female to male increased (P<0.05). T3 Treatment leading to 87.23% female. The use of plant extract increased the expression of gene CYP19 (a), so at the time of differentiation of gonad (45 days after hatching), the highest expression of this gene was occurred. Overall, suggest the use of T3 treatment to achieve the best reproductive performance and a higher percentage of females in zebrafish as model for aquaculture species.

Cites

No record.

References

No record.

Cite

APA: Copy

ENAYAT GHOLAMPOUR, TAYEBEH, JAFARI, VALIOLAH, IMANPOUR, MOHAMAD REZA, & KOLANGI MIANDARE, HAMED. (2016). INVESTIGATION OF EXPRESSION OF CYP19 (A) AND REPRODUCTION IN DANIO RERIOFED A DIET ENRICHED WITH VITEX AGNUS CASTUS EXTRACT. AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY, 4(3), 27-42. SID. https://sid.ir/paper/261273/en

Vancouver: Copy

ENAYAT GHOLAMPOUR TAYEBEH, JAFARI VALIOLAH, IMANPOUR MOHAMAD REZA, KOLANGI MIANDARE HAMED. INVESTIGATION OF EXPRESSION OF CYP19 (A) AND REPRODUCTION IN DANIO RERIOFED A DIET ENRICHED WITH VITEX AGNUS CASTUS EXTRACT. AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY[Internet]. 2016;4(3):27-42. Available from: https://sid.ir/paper/261273/en

IEEE: Copy

TAYEBEH ENAYAT GHOLAMPOUR, VALIOLAH JAFARI, MOHAMAD REZA IMANPOUR, and HAMED KOLANGI MIANDARE, “INVESTIGATION OF EXPRESSION OF CYP19 (A) AND REPRODUCTION IN DANIO RERIOFED A DIET ENRICHED WITH VITEX AGNUS CASTUS EXTRACT,” AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY, vol. 4, no. 3, pp. 27–42, 2016, [Online]. Available: https://sid.ir/paper/261273/en

Related Journal Papers