ANTICANCER EFFECTS OF LAURENCIA CASPICA EXTRACT ON BREASTCANCER T47D CELL LINE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SHAFAGHI MOJDEH; SALEHZADEH ALI; MOSHFEGH AZAM

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY Year:2016 | Volume:4 | Issue:1 Page(s): 69-83

Download Full-Text

Persian Verion

View:

1,858

Download:

Cites:

Information Journal Paper

Title

ANTICANCER EFFECTS OF LAURENCIA CASPICA EXTRACT ON BREASTCANCER T47D CELL LINE

Author(s)

SHAFAGHI MOJDEH | SALEHZADEH ALI | MOSHFEGH AZAM | Issue Writer Certificate

Keywords

LAURENCIA CASPICAQ2

NM23Q1

BREAST CANCERQ1

T47D CELL LINEQ1

Abstract

In order to treat cancer many drugs and natural products from different plants and animals are used. LAURENCIA CASPICA is one of the macroalgae of the Caspian Sea ecosystem. In the present study the toxicity effect and changes innm23 metastatic gene expression treated with L. caspica extract was evaluated.L. caspica alga was collected from the Ramsar beach and its hydroalcholic extracts were prepared via percolation method in 50% methanol. The breastcancer T47D CELL LINE was treated with concentrations of 25, 50, 100, 200, 400and 800mg/ml of extract. MTT assay was used to analyze extracts lethality.RNA was extracted and cDNA was synthesized. The specific primers were designed for NM23 and B-actin genes. Level of NM23 gene expression was compared to B-actin reference gene in the presence of the estimated IC50concentration using Quantitative Real Time PCR. Evaluation of cell survivalusing MTT assay indicated that L. caspica extract reduced the T47D cell growth in a dose dependent manner and also the 175mg/mL concentration of extract hadinhibitory effect on 50% of cells. Real Time PCR showed that the level of NM23metastatic gene expression had a significant increase, while the B-actin gene expression had not changed.

Cites

No record.

References

No record.

Cite

APA: Copy

SHAFAGHI, MOJDEH, SALEHZADEH, ALI, & MOSHFEGH, AZAM. (2016). ANTICANCER EFFECTS OF LAURENCIA CASPICA EXTRACT ON BREASTCANCER T47D CELL LINE. AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY, 4(1), 69-83. SID. https://sid.ir/paper/261323/en

Vancouver: Copy

SHAFAGHI MOJDEH, SALEHZADEH ALI, MOSHFEGH AZAM. ANTICANCER EFFECTS OF LAURENCIA CASPICA EXTRACT ON BREASTCANCER T47D CELL LINE. AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY[Internet]. 2016;4(1):69-83. Available from: https://sid.ir/paper/261323/en

IEEE: Copy

MOJDEH SHAFAGHI, ALI SALEHZADEH, and AZAM MOSHFEGH, “ANTICANCER EFFECTS OF LAURENCIA CASPICA EXTRACT ON BREASTCANCER T47D CELL LINE,” AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY, vol. 4, no. 1, pp. 69–83, 2016, [Online]. Available: https://sid.ir/paper/261323/en

Related Journal Papers