EFFECT OF THE CRYOPROTECTANTS GLYCEROL, ETHYLENE GLYCOL AND ACETAMIDE ON HATCHING RATE OF COMMON CARP (CYPRINUS CARPIO) EMBRYOS

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SUDAGAR MOHAMMAD; KEIVANLOO SAEIDE; MAZANDARANI MOHAMMAD

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Journal: AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY Year:2017 | Volume:5 | Issue:2 Page(s): 35-51

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Title

EFFECT OF THE CRYOPROTECTANTS GLYCEROL, ETHYLENE GLYCOL AND ACETAMIDE ON HATCHING RATE OF COMMON CARP (CYPRINUS CARPIO) EMBRYOS

Author(s)

SUDAGAR MOHAMMAD | KEIVANLOO SAEIDE | MAZANDARANI MOHAMMAD | Issue Writer Certificate

Keywords

CRYOPROTECTANTSQ1

EMBRYOQ1

CYPRINUS CARPIOQ2

TOXICITYQ1

HATCHING RATEQ2

Abstract

Fish EMBRYO cryopreservation means substantiation of the body water with a cryoprotectant. Knowledge of CRYOPROTECTANTS TOXICITY is one of the prerequisites for the design of EMBRYO cryopreservation protocols. In the present study common carps (Cyprinus carpio) in two stages of EMBRYOnic development, half-epiboly (8 h post-fertilization) and heartbeat (32 h post-fertilization) stage, were exposed to pronase, type XIV of Streptomyces griseus, for 5 min. Permeabilized EMBRYOs were immersed in various CRYOPROTECTANTS including glycerol, ethylene glycol and acetamide in concentrations of 1, 2, 3 and 4M for a period of 5 and 15min. EMBRYOs were then washed and incubated until hatched. The TOXICITY of the cryoprotectant was assessed by the HATCHING RATE. The results indicated that glycerol had less TOXICITY compared to ethylene glycol and acetamide. Moreover, EMBRYOs could tolerate glycerol in high concentrations. Acetamide was found to be the most toxic cryoprotectant. Reduced HATCHING RATE may be a result of osmotic shock, ionic imbalance or consequence of cryoprotectant TOXICITY. As concentrations were increased, HATCHING RATE decreased. Similarly, HATCHING RATEs of EMBRYOs were decreased as the exposure time increased (5 to 15 min). With the increasing of the EMBRYOnic development sensitivity to cryoprotectant were decreased. Results suggest that glycerol and ethylene glycol would be good options for cryopreservation of common carp EMBRYOs at heartbeat stage.

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APA: Copy

SUDAGAR, MOHAMMAD, KEIVANLOO, SAEIDE, & MAZANDARANI, MOHAMMAD. (2017). EFFECT OF THE CRYOPROTECTANTS GLYCEROL, ETHYLENE GLYCOL AND ACETAMIDE ON HATCHING RATE OF COMMON CARP (CYPRINUS CARPIO) EMBRYOS. AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY, 5(2), 35-51. SID. https://sid.ir/paper/261328/en

Vancouver: Copy

SUDAGAR MOHAMMAD, KEIVANLOO SAEIDE, MAZANDARANI MOHAMMAD. EFFECT OF THE CRYOPROTECTANTS GLYCEROL, ETHYLENE GLYCOL AND ACETAMIDE ON HATCHING RATE OF COMMON CARP (CYPRINUS CARPIO) EMBRYOS. AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY[Internet]. 2017;5(2):35-51. Available from: https://sid.ir/paper/261328/en

IEEE: Copy

MOHAMMAD SUDAGAR, SAEIDE KEIVANLOO, and MOHAMMAD MAZANDARANI, “EFFECT OF THE CRYOPROTECTANTS GLYCEROL, ETHYLENE GLYCOL AND ACETAMIDE ON HATCHING RATE OF COMMON CARP (CYPRINUS CARPIO) EMBRYOS,” AQUATIC PHYSIOLOGY AND BIOTECHNOLOGY, vol. 5, no. 2, pp. 35–51, 2017, [Online]. Available: https://sid.ir/paper/261328/en

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