Ultrasound effects on the activity of free and immobilized urease on the magnetic nanoparticles and silica gel

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Zahedi Salangooch f.; HOUSAINDOKHT M.R.; JALAL R.; NAKHAEIPOUR A.; IZADI NAJAFABADI R.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF ACOUSTICAL ENGINEERING SOCIETY OF IRAN Year:2019 | Volume:6 | Issue:2 Page(s): 9-20

Download Full-Text

Persian Verion

View:

416

Download:

Cites:

Information Journal Paper

Title

Ultrasound effects on the activity of free and immobilized urease on the magnetic nanoparticles and silica gel

Author(s)

Keywords

Urease

Ultrasound wave

Enzyme immobilization

Fe2O3 magnetic nanoparticle

Silica gel

Abstract

In this study, the effect of low-frequency ultrasound on the structure and activity of Urease enzyme has been investigated. For this purpose, the enzyme was exposed to ultrasound with 20 kHz frequency at different time intervals. The Urease enzyme was immobilized on the surface of magnetic nanoparticles (Fe2O3), and activated Silica gel with 89% and 66% efficiency respectively. The enzyme activity, kinetic parameters, optimum pH and temperature were determined for free and immobilized enzymes at different conditions. The structure change of free enzymes caused by ultrasound was vreified by fluorescence and UV spectrum. The activity of enzyme was amplified in the presence of ultrasound. A reduction in the activity and Vmax and an increase in Km were observed for both free and immobilized enzymes on the activated Silica gel after ultrasound treatment. While, ultrasound had no effect on the immobilized enzymes on the magnetic nanoparticles.

Cites

No record.

References

No record.

Cite

APA: Copy

Zahedi Salangooch, f., HOUSAINDOKHT, M.R., JALAL, R., NAKHAEIPOUR, A., & IZADI NAJAFABADI, R.. (2019). Ultrasound effects on the activity of free and immobilized urease on the magnetic nanoparticles and silica gel. JOURNAL OF ACOUSTICAL ENGINEERING SOCIETY OF IRAN, 6(2 ), 9-20. SID. https://sid.ir/paper/268656/en

Vancouver: Copy

Zahedi Salangooch f., HOUSAINDOKHT M.R., JALAL R., NAKHAEIPOUR A., IZADI NAJAFABADI R.. Ultrasound effects on the activity of free and immobilized urease on the magnetic nanoparticles and silica gel. JOURNAL OF ACOUSTICAL ENGINEERING SOCIETY OF IRAN[Internet]. 2019;6(2 ):9-20. Available from: https://sid.ir/paper/268656/en

IEEE: Copy

f. Zahedi Salangooch, M.R. HOUSAINDOKHT, R. JALAL, A. NAKHAEIPOUR, and R. IZADI NAJAFABADI, “Ultrasound effects on the activity of free and immobilized urease on the magnetic nanoparticles and silica gel,” JOURNAL OF ACOUSTICAL ENGINEERING SOCIETY OF IRAN, vol. 6, no. 2 , pp. 9–20, 2019, [Online]. Available: https://sid.ir/paper/268656/en

Related Journal Papers