HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATION OF SODIUM BENZOATE, METHYLPARABEN AND PROPYLPARABEN AS PRESERVATIVE COMPONENTS IN NYSTATIN SUSPENSIONS

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ABD ELAHPOUR A.; FOROUHI M.; SHAMSIPOUR M.; YAMINI Y.

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Paper Information

Journal: JOURNAL OF THE IRANIAN CHEMICAL SOCIETY(JICS) Year:2010 | Volume:7 | Issue:2 Page(s): 516-520

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Title

HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATION OF SODIUM BENZOATE, METHYLPARABEN AND PROPYLPARABEN AS PRESERVATIVE COMPONENTS IN NYSTATIN SUSPENSIONS

Author(s)

ABD ELAHPOUR A. | FOROUHI M. | SHAMSIPOUR M. | YAMINI Y. | Issue Writer Certificate

Keywords

PRESERVATIVE

DETERMINATION

NYSTATIN

REVERSED PHASE

HPLC

Abstract

A simple and sensitive method was developed for the analysis of PRESERVATIVEs sodium benzoate, methylparaben and propylparaben in NYSTATIN suspensions by reversed-phase high performance liquid chromatography (HPLC), equipped with a C18 column and PDA detector. The mobile phase was a mixture of acetonitrile and acetate buffer of pH 4.4 (35:65 v/v). Under the optimized experimental conditions, separation of the PRESERVATIVEs was achieved in less than 20 min. The limits of quantifications (LOQs) and the linear dynamic ranges (LDRs) of sodium benzoate, methylparaben and propylparaben were 0.3 and 50-1000 µg ml-1, 0.5 and 50-600 µg ml-1 and 0.3 and 50-900 µg ml-1, respectively; the respective precisions (%RSD) at 500 µg ml-1 level were 0.72%, 0.73% and 0.51% (n=6). The average recoveries of sodium benzoate, methylparaben and propylparaben for spiked NYSTATIN samples were obtained as 98%, 97% and 98%, respectively.

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APA: Copy

ABD ELAHPOUR, A., FOROUHI, M., SHAMSIPOUR, M., & YAMINI, Y.. (2010). HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATION OF SODIUM BENZOATE, METHYLPARABEN AND PROPYLPARABEN AS PRESERVATIVE COMPONENTS IN NYSTATIN SUSPENSIONS. JOURNAL OF THE IRANIAN CHEMICAL SOCIETY(JICS), 7(2), 516-520. SID. https://sid.ir/paper/282371/en

Vancouver: Copy

ABD ELAHPOUR A., FOROUHI M., SHAMSIPOUR M., YAMINI Y.. HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATION OF SODIUM BENZOATE, METHYLPARABEN AND PROPYLPARABEN AS PRESERVATIVE COMPONENTS IN NYSTATIN SUSPENSIONS. JOURNAL OF THE IRANIAN CHEMICAL SOCIETY(JICS)[Internet]. 2010;7(2):516-520. Available from: https://sid.ir/paper/282371/en

IEEE: Copy

A. ABD ELAHPOUR, M. FOROUHI, M. SHAMSIPOUR, and Y. YAMINI, “HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATION OF SODIUM BENZOATE, METHYLPARABEN AND PROPYLPARABEN AS PRESERVATIVE COMPONENTS IN NYSTATIN SUSPENSIONS,” JOURNAL OF THE IRANIAN CHEMICAL SOCIETY(JICS), vol. 7, no. 2, pp. 516–520, 2010, [Online]. Available: https://sid.ir/paper/282371/en

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