THE RELEVANCE OF LOW-AFFINITY CA-BINDING SITES TO THE STRUCTURE, STABILITY AND KINETIC PROPERTIES OF THERMOLYSIN

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ASGHARI S.MOHSEN; ISAZADEH MOHSEN; TAGHDIR MAJID

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Journal: JOURNAL OF THE IRANIAN CHEMICAL SOCIETY(JICS) Year:2016 | Volume:13 | Issue:10 Page(s): 0-0

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Title

THE RELEVANCE OF LOW-AFFINITY CA-BINDING SITES TO THE STRUCTURE, STABILITY AND KINETIC PROPERTIES OF THERMOLYSIN

Author(s)

ASGHARI S.MOHSEN | ISAZADEH MOHSEN | TAGHDIR MAJID | Issue Writer Certificate

Keywords

THERMOLYSIN CA-BINDINGSTRUCTUREENZYME

KINETICSSTABILITY

Abstract

To clarify the significance of low-affinity Ca-binding sites in thermolysin (TLN), we compared the enzymes having bound and free weak Ca-binding sites. Binding measurements suggested that TLN comprises two strong and two weak Ca-binding sites. Far-UV CD analysis indicated that the weak sites do not affect TLN’s secondary structure. However, a slight local rearrangement due to the weak sites’ occupancy was deduced by the fluorescence measurements. Upon Ca-binding to weak sites, KM and kcat for hydrolysis of 3-(-2-furylacryloyl)-L-glycyl-L-leucine-amide were reduced about 2.8- and 1.8-fold, respectively, and kcat/KM was therefore improved about 1.5-fold. Our modeling studies suggest that the modified kinetic properties may be due to altered hinge angle between N- and C-terminal domains upon Ca-binding to the weak sites. TLN exhibited a higher temperature optimum, remarkable increase in the half-life of thermoinactivation t1/2 and enhanced remaining activity in the presence of denaturant agents in a Ca-dependent manner. In contrast, organic solvents and pH stabilities were irrelevant to the weak sites. Finally, we conclude that low-affinity sites of TLN may induce subtle changes in the local environment and modulate the enzymatic activity and thermal stability.

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APA: Copy

ASGHARI, S.MOHSEN, ISAZADEH, MOHSEN, & TAGHDIR, MAJID. (2016). THE RELEVANCE OF LOW-AFFINITY CA-BINDING SITES TO THE STRUCTURE, STABILITY AND KINETIC PROPERTIES OF THERMOLYSIN. JOURNAL OF THE IRANIAN CHEMICAL SOCIETY(JICS), 13(10), 0-0. SID. https://sid.ir/paper/283244/en

Vancouver: Copy

ASGHARI S.MOHSEN, ISAZADEH MOHSEN, TAGHDIR MAJID. THE RELEVANCE OF LOW-AFFINITY CA-BINDING SITES TO THE STRUCTURE, STABILITY AND KINETIC PROPERTIES OF THERMOLYSIN. JOURNAL OF THE IRANIAN CHEMICAL SOCIETY(JICS)[Internet]. 2016;13(10):0-0. Available from: https://sid.ir/paper/283244/en

IEEE: Copy

S.MOHSEN ASGHARI, MOHSEN ISAZADEH, and MAJID TAGHDIR, “THE RELEVANCE OF LOW-AFFINITY CA-BINDING SITES TO THE STRUCTURE, STABILITY AND KINETIC PROPERTIES OF THERMOLYSIN,” JOURNAL OF THE IRANIAN CHEMICAL SOCIETY(JICS), vol. 13, no. 10, pp. 0–0, 2016, [Online]. Available: https://sid.ir/paper/283244/en

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