EVALUATION OF GENETIC DIVERSITY IN EAST AZARBAIJAN AND FARS PROVINCES AMONG RALSTONIA SOLANACEARUM ISOLATES BY REP-PCR

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IRANDOUST H.; NIKNAM GH.R.; GHASEMI A.A.GH.; TAGHAVI SEYED MOHSEN; TORABI E.

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Journal: JOURNAL OF AGRICULTURAL KNOWLEDGE Year:2008 | Volume:18 | Issue:3 Page(s): 233-242

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Title

EVALUATION OF GENETIC DIVERSITY IN EAST AZARBAIJAN AND FARS PROVINCES AMONG RALSTONIA SOLANACEARUM ISOLATES BY REP-PCR

Author(s)

Keywords

BIOVAR 2AQ3

BIOVAR N2Q3

RALSTONIA SOLANACEARUMQ3

REP-PCRQ3

Abstract

In order to study genetic diversity among East Azarbaijan and Fars provinces isolates of RALSTONIA SOLANACEARUM, samples were collected from potato fields. A total of 35 bacterial isolates from East Azarbaijan province, three isolates from Fars province and one isolate from infected tubers of Karaj market were isolated from potato samples. DNA fingerprinting with two REP-PCR primers (BOX and Rep) was performanced on selected isolates including five isolates from East Azarbaijan, three from Fars, one from Karaj market and two from Costa Rica as standard isolate. The length of produced fragments ranged from 200 bp to 1200 bp in BOX primer and 200 bp to 1500 bp in Rep primer. The grouping of isolates using UPGMA method and Jaccard's coefficient which obtained from combined molecular data of the both primers grouped the isolates of R. solanacearum in two distinguished clusters. Group one included Fars isolates BIOVAR N2 and group two consisted of eight BIOVAR 2A. The results of this study indicated that REP-PCR technique with two Rep and BOX primers is capable to show differences among biovars 2A and between BIOVAR 2A and BIOVAR N2 as well as genetic polymorphism among different populations of R. solanacearum.

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APA: Copy

IRANDOUST, H., NIKNAM, GH.R., GHASEMI, A.A.GH., TAGHAVI, SEYED MOHSEN, & TORABI, E.. (2008). EVALUATION OF GENETIC DIVERSITY IN EAST AZARBAIJAN AND FARS PROVINCES AMONG RALSTONIA SOLANACEARUM ISOLATES BY REP-PCR. JOURNAL OF AGRICULTURAL KNOWLEDGE, 18(3), 233-242. SID. https://sid.ir/paper/28923/en

Vancouver: Copy

IRANDOUST H., NIKNAM GH.R., GHASEMI A.A.GH., TAGHAVI SEYED MOHSEN, TORABI E.. EVALUATION OF GENETIC DIVERSITY IN EAST AZARBAIJAN AND FARS PROVINCES AMONG RALSTONIA SOLANACEARUM ISOLATES BY REP-PCR. JOURNAL OF AGRICULTURAL KNOWLEDGE[Internet]. 2008;18(3):233-242. Available from: https://sid.ir/paper/28923/en

IEEE: Copy

H. IRANDOUST, GH.R. NIKNAM, A.A.GH. GHASEMI, SEYED MOHSEN TAGHAVI, and E. TORABI, “EVALUATION OF GENETIC DIVERSITY IN EAST AZARBAIJAN AND FARS PROVINCES AMONG RALSTONIA SOLANACEARUM ISOLATES BY REP-PCR,” JOURNAL OF AGRICULTURAL KNOWLEDGE, vol. 18, no. 3, pp. 233–242, 2008, [Online]. Available: https://sid.ir/paper/28923/en

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