مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

REDUCTION OF NADH OXIDASE, NO SYNTHASE, TNFα, AND IL-1β MRNA EXPRESSION LEVELS ON LIPOPOLYSACHARIDE-STIMULATED MURINE MACROPHAGES BY ZATARIA MULTIFLORA

Pages

  89-100

Abstract

 Zataria multiflora (ZM) is a thyme-like aromatic plant in the Lamiaceae family that grows in central and southern Iran. ZM is extensively used as a flavor ingredient in a wide variety of foods and is used as part of popular traditional folk remedies. In the present study, ZM essential oil (ZMO) was obtained from ZM leaves via hydro-distillation and then analyzed by GC-MS (gas chromatography-mass spectrometry). The anti-inflammatory activity of ZMO was determined via measures of NADH OXIDASE (NOX), inducible nitric oxide synthase (iNOS), tumor necrosis factor (TNF)-a, and interleukin (IL)-1bmRNA expression in lipopolysaccharide-stimulated murine MACROPHAGES using real-time polymerase chain reaction (PCR). GC-MS analysis indicated that the main components in the ZMO were carvacrol (29.4%), thymol (25.7%), p-cymene (11.2%), linalool (9.3%), and g-terpinene (8.0%). ZMO significantly reduced NOX, iNOS, TNFa, and IL-1bmRNA expression in cells at concentrations of 0.1-1 m/mL, indicating a capacity for this product to potentially modulate/diminish immune responses. ZMO has anti-oxidant and anti-inflammatory properties and could be potentially used as a safe effective source of natural anti-oxidants in therapy against oxidative damage and a number of inflammatory conditions associated with stress.

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    Cite

    APA: Copy

    KARIMIAN, PARASTOO, KAVOOSI, GHOLAMREZA, AMIRGHOFRAN, ZAHRA, & KALANTAR, FATHOLLA. (2013). REDUCTION OF NADH OXIDASE, NO SYNTHASE, TNFα, AND IL-1β MRNA EXPRESSION LEVELS ON LIPOPOLYSACHARIDE-STIMULATED MURINE MACROPHAGES BY ZATARIA MULTIFLORA. MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS, 2(3), 89-100. SID. https://sid.ir/paper/335844/en

    Vancouver: Copy

    KARIMIAN PARASTOO, KAVOOSI GHOLAMREZA, AMIRGHOFRAN ZAHRA, KALANTAR FATHOLLA. REDUCTION OF NADH OXIDASE, NO SYNTHASE, TNFα, AND IL-1β MRNA EXPRESSION LEVELS ON LIPOPOLYSACHARIDE-STIMULATED MURINE MACROPHAGES BY ZATARIA MULTIFLORA. MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS[Internet]. 2013;2(3):89-100. Available from: https://sid.ir/paper/335844/en

    IEEE: Copy

    PARASTOO KARIMIAN, GHOLAMREZA KAVOOSI, ZAHRA AMIRGHOFRAN, and FATHOLLA KALANTAR, “REDUCTION OF NADH OXIDASE, NO SYNTHASE, TNFα, AND IL-1β MRNA EXPRESSION LEVELS ON LIPOPOLYSACHARIDE-STIMULATED MURINE MACROPHAGES BY ZATARIA MULTIFLORA,” MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS, vol. 2, no. 3, pp. 89–100, 2013, [Online]. Available: https://sid.ir/paper/335844/en

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