FIREFLY LUCIFERASE COMPLEMENTATION ASSAY FOR IP3 DETECTION

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ATAEI FARANGIS; TORKZADEH MAHANI MASOUD; HOSSEINKHANI SAMAN

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Journal Paper

Paper Information

Journal: MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS Year:2014 | Volume:3 | Issue:SUPPL. (1) Page(s): 35-35

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Information Journal Paper

Title

FIREFLY LUCIFERASE COMPLEMENTATION ASSAY FOR IP3 DETECTION

Author(s)

ATAEI FARANGIS | TORKZADEH MAHANI MASOUD | HOSSEINKHANI SAMAN

Keywords

LUCIFERASE

IP3

BIOSENSOR

Abstract

LUCIFERASE is the key enzyme that catalyzes the oxygenation of luciferin, generating energy-rich peroxidic intermediates, emitting a photon of visible light. Inositol 1, 4, 5- trisphosphate (IP3) plays a vital role in the control of a large number of cellular processes.Alteration in its content has been observed in metabolic disorders. Hence, development of high-throughput screening systems to monitor temporal changes of IP3 is essential for screening of new potential therapeutic compounds. Toward a sensitive, rapid and simple method to measuring IP3, we describe the development and application of a LUCIFERASEfragment complementation strategy, which converts the ligand-induced conformational changes to light. In order to create a good performance sensor for IP3 monitoring, IP3 binding domain (IP3BD) of IP3R that consist of all the specific binding site of IP3 was selected as a ligand binding domain. The NLuc of firefly LUCIFERASE is fused to the Nterminus of IP3BD whiles the CLuc of LUCIFERASE is attached to the C-terminus of IP3BD (NLuc-IP3BD-CLuc). A flexible peptide linker (G4S) 2 was inserted between the fragmented LUCIFERASE and IP3BD to assist in proper protein folding and LUCIFERASE complementation. According to the results, the screening time was very fast and maximum response was obtained up to 11-fold higher than background. Moreover, the designed BIOSENSOR was able to monitor release of IP3 upon induction by different inducer like Bradykinin and ATP. The current BIOSENSOR not only provides a specific IP3 detector in vitro, but also facilitates to monitoring of the response of IP3 in living organisms.

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APA: Copy

ATAEI, FARANGIS, TORKZADEH MAHANI, MASOUD, & HOSSEINKHANI, SAMAN. (2014). FIREFLY LUCIFERASE COMPLEMENTATION ASSAY FOR IP3 DETECTION. MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS, 3(SUPPL. (1)), 35-35. SID. https://sid.ir/paper/335899/en

Vancouver: Copy

ATAEI FARANGIS, TORKZADEH MAHANI MASOUD, HOSSEINKHANI SAMAN. FIREFLY LUCIFERASE COMPLEMENTATION ASSAY FOR IP3 DETECTION. MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS[Internet]. 2014;3(SUPPL. (1)):35-35. Available from: https://sid.ir/paper/335899/en

IEEE: Copy

FARANGIS ATAEI, MASOUD TORKZADEH MAHANI, and SAMAN HOSSEINKHANI, “FIREFLY LUCIFERASE COMPLEMENTATION ASSAY FOR IP3 DETECTION,” MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS, vol. 3, no. SUPPL. (1), pp. 35–35, 2014, [Online]. Available: https://sid.ir/paper/335899/en

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