PROTEIN EXTRACTION FROM HUMAN BRAIN FRESH-FROZEN TISSUE FOR SEPARATION BY ELECTROPHORESIS

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POOLADI MEHDI

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Journal Paper

Paper Information

Journal: MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS Year:2014 | Volume:3 | Issue:SUPPL. (1) Page(s): 245-245

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Title

PROTEIN EXTRACTION FROM HUMAN BRAIN FRESH-FROZEN TISSUE FOR SEPARATION BY ELECTROPHORESIS

Author(s)

POOLADI MEHDI

Keywords

BRAIN

PROTEIN EXTRACTION

FROZEN TISSUE

Abstract

Proteins from frozen tissue samples need to be extracted efficiently and without degradation to make the best use of a limited resource and to ensure, as much as possible, that an accurate representation of the proteins in the living tissue is obtained. The emergence of powerful proteomic techniques that make possible studies of many proteins simultaneously and the possibility of automated dispensations from biobank facilities have refocused attention on optimized. Tissues were obtained, with informed consent and institutional review board approval, from patients undergoing tumor resection. For this study, all individuals filled a written informed consent form tissue were surgically removed at hospital. Tissue samples of both tumoral and normal brain tissue were snap-frozen immediately after operation in liquid nitrogen and stored at -80oC until used for proteomic analysis. We extracted proteins of tumor and normal brain tissues and then evaluated the protein purity by spectrophotometry method. The first-dimensional electrophoresis was performed using 18 cm, pH 3–10 IPG strips. The total number of protein features was matched and analyzed between gels in the control group and tumor group; 343 spots (around 49% of the entire detected spots) were matched across all the gels. In software analysis, a total of 343 differentially expressed spots satisfied the statistical parameters (p <0.05). Efficient extraction is in fact, in part, dependent on breaking protein interactions to release proteins bound in macromolecular assemblies. Disruptive methods of solubilization can, and should, therefore be applied, short of hydrolyzing the protein amino-acid chain or posttranslational modifications.

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APA: Copy

POOLADI, MEHDI. (2014). PROTEIN EXTRACTION FROM HUMAN BRAIN FRESH-FROZEN TISSUE FOR SEPARATION BY ELECTROPHORESIS. MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS, 3(SUPPL. (1)), 245-245. SID. https://sid.ir/paper/335943/en

Vancouver: Copy

POOLADI MEHDI. PROTEIN EXTRACTION FROM HUMAN BRAIN FRESH-FROZEN TISSUE FOR SEPARATION BY ELECTROPHORESIS. MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS[Internet]. 2014;3(SUPPL. (1)):245-245. Available from: https://sid.ir/paper/335943/en

IEEE: Copy

MEHDI POOLADI, “PROTEIN EXTRACTION FROM HUMAN BRAIN FRESH-FROZEN TISSUE FOR SEPARATION BY ELECTROPHORESIS,” MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS, vol. 3, no. SUPPL. (1), pp. 245–245, 2014, [Online]. Available: https://sid.ir/paper/335943/en

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