NANOCOMPLEXES ARISING FROM PROTEIN-POLYSACCHARIDE ASSOCIATIVE PHASE SEPARATION AS A PROMISING VEHICLE FOR BIOACTIVE COMPOUNDS

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HOSSEINI SEYED MOHAMMAD HASHEM; EMAM DJOMEH ZAHRA

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Journal Paper

Paper Information

Journal: MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS Year:2014 | Volume:3 | Issue:SUPPL. (1) Page(s): 168-168

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Title

NANOCOMPLEXES ARISING FROM PROTEIN-POLYSACCHARIDE ASSOCIATIVE PHASE SEPARATION AS A PROMISING VEHICLE FOR BIOACTIVE COMPOUNDS

Author(s)

HOSSEINI SEYED MOHAMMAD HASHEM | EMAM DJOMEH ZAHRA

Keywords

COACERVATION

BETA-LACTOGLOBULIN

NANOSCOPIC DELIVERY SYSTEM

Abstract

Most of the nutraceuticals such as carotenoids, fat-soluble vitamins and phenolic compounds show instability against chemical or physical degradation and tend to degrade during processing or storage when incorporated into foods. Among different types of food products, incorporating bioactives into non-fat aqueous foods and beverages (especially clear ones) is challenging due to the hydrophobic nature of most bioactives and their instability. The main purpose of the current work was exploring the potential application of the protein-polysaccharide soluble nanocomplexes as delivery systems for nutraceuticals in liquid foods. In this study, the intrinsic transporting property of b-lactoglobulin (BLG) was utilized to develop nanoscale green delivery systems. The binding analysis using fluorescence spectroscopy suggested that the complexation between BLG and four nutraceutical models including b-carotene, folic acid, curcumin and ergocalciferol occurred under all conditions but varied as a function of pH and nutraceutical type. The 1H-NMR study of hydrophilic ligands binding to BLG provided complementary information on the interactions between protein and water soluble ligands. These findings resulted in designing nanoscopic delivery systems for encapsulation of both hydrophobic and hydrophilic bioactives in clear liquid food products of acidic pH. The stability experiments demonstrated that the nutraceuticals of low solubility in water were successfully entrapped within electrostatically stable nanocomplexes arising from BLG-sodium alginate interactions. The electrophoretic mobility analysis showed that soluble nanocomplexes had good stability against aggregation.

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APA: Copy

HOSSEINI, SEYED MOHAMMAD HASHEM, & EMAM DJOMEH, ZAHRA. (2014). NANOCOMPLEXES ARISING FROM PROTEIN-POLYSACCHARIDE ASSOCIATIVE PHASE SEPARATION AS A PROMISING VEHICLE FOR BIOACTIVE COMPOUNDS. MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS, 3(SUPPL. (1)), 168-168. SID. https://sid.ir/paper/336020/en

Vancouver: Copy

HOSSEINI SEYED MOHAMMAD HASHEM, EMAM DJOMEH ZAHRA. NANOCOMPLEXES ARISING FROM PROTEIN-POLYSACCHARIDE ASSOCIATIVE PHASE SEPARATION AS A PROMISING VEHICLE FOR BIOACTIVE COMPOUNDS. MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS[Internet]. 2014;3(SUPPL. (1)):168-168. Available from: https://sid.ir/paper/336020/en

IEEE: Copy

SEYED MOHAMMAD HASHEM HOSSEINI, and ZAHRA EMAM DJOMEH, “NANOCOMPLEXES ARISING FROM PROTEIN-POLYSACCHARIDE ASSOCIATIVE PHASE SEPARATION AS A PROMISING VEHICLE FOR BIOACTIVE COMPOUNDS,” MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS, vol. 3, no. SUPPL. (1), pp. 168–168, 2014, [Online]. Available: https://sid.ir/paper/336020/en

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