IN VITRO MATURATION OF DROMEDARY CAMEL OOCYTES IN HAMS'S F10 MEDIUM SUPPLEMENTED WITH DIFFERENT CONCENTRATIONS OF FETAL BOVINE SERUM

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TAJIK P.; POUYAN H.R.; ZARE SHAHNEH A.R.; GHASEMZADEH NAVA H.; MIR SHOKRAEI P.

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Journal: Journal of Veterinary Research Year:2005 | Volume:60 | Issue:2 Page(s): 143-148

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Title

IN VITRO MATURATION OF DROMEDARY CAMEL OOCYTES IN HAMS'S F10 MEDIUM SUPPLEMENTED WITH DIFFERENT CONCENTRATIONS OF FETAL BOVINE SERUM

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Keywords

IN VITRO MATURATIONQ2

DROMEDARY CAMELQ3

HAM'S F10Q4

FBSQ4

COOL STORAGEQ4

Abstract

Objectives: To mature DROMEDARY CAMEL oocytes for using them in an IVF system. Design: Interventional study. Animals: Ovaries from DROMEDARY CAMELs in local slaughterhouses. Procedure: Removing varies from camels in a local slaughterhouse, carrying them to the laboratory in warm saline solution, aspiration of follicles, isolation and transferring of oocytes into TCM-199 and HAM'S F10 supplemented with 0-10% heat inactivated fetal bovine serum (FBS), culturing oocytes for up to 24h in a CO2 incubator. After culture oocytes were denuded and put into PBS containing 0.1% hyaluronidase and passing through a fine pipette. Oocytes were then mounted onto slide glass and fixed and stained for evidence of maturation. Statistical analysis: ANOVA and when significance different was seen, Duncan's Multiple Range Test. Results: When oocytes from fresh ovaries were culture in HAM'S F10 without protein, only 17.65% of them reached to MH. However, significantly (P<0.05) higher oocytes reached to MH in 5 and 10% FCS (36.84% and 33.33% for 5 and 10% FBS respectively), which were not dose dependent. When cool stored ovaries were used for oocyte maturation, 14.54% of oocytes reached to MH. In protein-free medium However, significantly (P<0.05) higher oocytes reached to MH in 5 and 10% FCS (25.86% and 33.33% for 5 and 10% FBS respectively). Although increasing the protein increased the maturation rates, the difference was not significant. Conclusion: Under the present condition it seems that cool stored ovaries could be used for IN VITRO MATURATION of camel oocytes.

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APA: Copy

TAJIK, P., POUYAN, H.R., ZARE SHAHNEH, A.R., GHASEMZADEH NAVA, H., & MIR SHOKRAEI, P.. (2005). IN VITRO MATURATION OF DROMEDARY CAMEL OOCYTES IN HAMS'S F10 MEDIUM SUPPLEMENTED WITH DIFFERENT CONCENTRATIONS OF FETAL BOVINE SERUM. JOURNAL OF VETERINARY RESEARCH, 60(2), 143-148. SID. https://sid.ir/paper/34666/en

Vancouver: Copy

TAJIK P., POUYAN H.R., ZARE SHAHNEH A.R., GHASEMZADEH NAVA H., MIR SHOKRAEI P.. IN VITRO MATURATION OF DROMEDARY CAMEL OOCYTES IN HAMS'S F10 MEDIUM SUPPLEMENTED WITH DIFFERENT CONCENTRATIONS OF FETAL BOVINE SERUM. JOURNAL OF VETERINARY RESEARCH[Internet]. 2005;60(2):143-148. Available from: https://sid.ir/paper/34666/en

IEEE: Copy

P. TAJIK, H.R. POUYAN, A.R. ZARE SHAHNEH, H. GHASEMZADEH NAVA, and P. MIR SHOKRAEI, “IN VITRO MATURATION OF DROMEDARY CAMEL OOCYTES IN HAMS'S F10 MEDIUM SUPPLEMENTED WITH DIFFERENT CONCENTRATIONS OF FETAL BOVINE SERUM,” JOURNAL OF VETERINARY RESEARCH, vol. 60, no. 2, pp. 143–148, 2005, [Online]. Available: https://sid.ir/paper/34666/en

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