A COMPARATIVE STUDY OF ACTIVITY AND STABILITY OF THE FREE AND THE IMMOBILIZED ENDOGLUCANASE FROM ALICYCLOBACILLUS ACIDOCALDARIUS

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RAHIMIZADEH P.; NAJAVAND S.; PAZHANG M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: BIOMACROMOLECULAR JOURNAL Year:2015 | Volume:1 | Issue:2 Page(s): 167-176

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Title

A COMPARATIVE STUDY OF ACTIVITY AND STABILITY OF THE FREE AND THE IMMOBILIZED ENDOGLUCANASE FROM ALICYCLOBACILLUS ACIDOCALDARIUS

Author(s)

RAHIMIZADEH P. | NAJAVAND S. | PAZHANG M. | Issue Writer Certificate

Keywords

AACEL9A

IMMOBILIZATION

CHITOSAN

THERMAL STABILITY

Abstract

AACEL9A [b-1,4-endoglucanase, (E.C:3.2.1.4)], was immobilized onto glutaraldehyde activated CHITOSAN macrosphere by covalent attachment. The properties of the immobilized AACEL9A were investigated by determining the optimum pH and optimum temperature for activity, THERMAL STABILITY, and kinetic parameters. The IMMOBILIZATION process shifted the enzyme’s optimum temperature from 65oC for the free enzyme towards a wider temperature range from 60-80oC by the immobilized enzyme. The optimum pH of immobilized AACEL9A shifted to basic pH (pH 8) relative to free AACEL9A (pH 6.5). The IMMOBILIZATION on CHITOSAN macrosphere enhanced half-life of AACEL9A enzyme. After 60 min, the immobilized and the free enzyme retained 75% and 40% their activity at 65oC, respectively. The immobilized enzyme showed higher THERMAL STABILITY than the free form. Km value of immobilized AACEL9A (17.05 mg ml-1) was higher than free AACEL9A (7.75 mg ml-1). Also, CMC hydrolysis by immobilized and free AACEL9A in the presence of SDS detergent was investigated. The results showed that the immobilized enzyme maintained its activity more than the free form in different concentrations of SDS.

Cites

IMMOBILIZATION OF INULINASE FROM ASPERGILLUS NIGER ON MAGNETIC NANOPEPTIDES OF WHEAT GLUTEN HYDROLYSATES FOR HIGH FRUCTOSE SYRUP PRODUCTION

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APA: Copy

RAHIMIZADEH, P., NAJAVAND, S., & PAZHANG, M.. (2015). A COMPARATIVE STUDY OF ACTIVITY AND STABILITY OF THE FREE AND THE IMMOBILIZED ENDOGLUCANASE FROM ALICYCLOBACILLUS ACIDOCALDARIUS. BIOMACROMOLECULAR JOURNAL, 1(2), 167-176. SID. https://sid.ir/paper/348328/en

Vancouver: Copy

RAHIMIZADEH P., NAJAVAND S., PAZHANG M.. A COMPARATIVE STUDY OF ACTIVITY AND STABILITY OF THE FREE AND THE IMMOBILIZED ENDOGLUCANASE FROM ALICYCLOBACILLUS ACIDOCALDARIUS. BIOMACROMOLECULAR JOURNAL[Internet]. 2015;1(2):167-176. Available from: https://sid.ir/paper/348328/en

IEEE: Copy

P. RAHIMIZADEH, S. NAJAVAND, and M. PAZHANG, “A COMPARATIVE STUDY OF ACTIVITY AND STABILITY OF THE FREE AND THE IMMOBILIZED ENDOGLUCANASE FROM ALICYCLOBACILLUS ACIDOCALDARIUS,” BIOMACROMOLECULAR JOURNAL, vol. 1, no. 2, pp. 167–176, 2015, [Online]. Available: https://sid.ir/paper/348328/en

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