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Information Journal Paper

Title

Prediction of the Presence of Lipid Derivatives in Follicular Fluid and Reproductive Outcome among Infertile Women by MALDI Mass Spectrometry Method

Pages

  285-293

Abstract

 It has been documented that specific Follicular fluid (FF) biochemical characteristics may be essential to determining oocyte quality. Lipid derivatives have the most important role in the fertilization process and embryonic development. MALDI mass spectrometry is used for the diagnosis of biomolecules in the FF and serum of infertile women. FF and blood samples from 13 infertile women (20-38 years old) undergoing intracytoplasmic sperm injection (ICSI) was obtained, stored frozen at-80 º C, and later analyzed for Lipid derivatives. Both FF and serum samples were compared and Mfg was used as similarity index. For comparison between the FF and serum samples for one person, the mass spectrometry (MS) intensity ratio versus molecular weight, between the FF and serum samples were calculated. Out of 13 patients, three ongoing pregnancies were observed, so the percentage of pregnancy in the studied population was 25%. The patients who became pregnant after micro-injection had higher unsaturated fatty acids and omega-3 fatty acid compared to other samples, but Stearic and palmitic fatty acids were not high in these patients. A number of specific fatty esters and peptide derivatives in the FF and serum samples were found that may help to improve oocyte quality.

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    APA: Copy

    MIRABI, PARVANEH, CHAICHI, MOHAMMAD JAVAD, ESMAEILZADEH, SEDIGHEH, Nabavi, Seyed Reza, JORSARAEI, SEYED GHOLAM ALI, & Ehsani, Mahjobeh. (2017). Prediction of the Presence of Lipid Derivatives in Follicular Fluid and Reproductive Outcome among Infertile Women by MALDI Mass Spectrometry Method. ANALYTICAL AND BIOANALYTICAL CHEMISTRY RESEARCH, 4(2), 285-293. SID. https://sid.ir/paper/354287/en

    Vancouver: Copy

    MIRABI PARVANEH, CHAICHI MOHAMMAD JAVAD, ESMAEILZADEH SEDIGHEH, Nabavi Seyed Reza, JORSARAEI SEYED GHOLAM ALI, Ehsani Mahjobeh. Prediction of the Presence of Lipid Derivatives in Follicular Fluid and Reproductive Outcome among Infertile Women by MALDI Mass Spectrometry Method. ANALYTICAL AND BIOANALYTICAL CHEMISTRY RESEARCH[Internet]. 2017;4(2):285-293. Available from: https://sid.ir/paper/354287/en

    IEEE: Copy

    PARVANEH MIRABI, MOHAMMAD JAVAD CHAICHI, SEDIGHEH ESMAEILZADEH, Seyed Reza Nabavi, SEYED GHOLAM ALI JORSARAEI, and Mahjobeh Ehsani, “Prediction of the Presence of Lipid Derivatives in Follicular Fluid and Reproductive Outcome among Infertile Women by MALDI Mass Spectrometry Method,” ANALYTICAL AND BIOANALYTICAL CHEMISTRY RESEARCH, vol. 4, no. 2, pp. 285–293, 2017, [Online]. Available: https://sid.ir/paper/354287/en

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