Using Boehmite Nanoparticles as an Undercoat, and Riboflavin as a Redox Probe for Immunosensor Designing: Ultrasensitive Detection of Hepatitis C Virus Core Antigen

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Valipour Akram; ROUSHANI MAHMOUD

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Journal Paper

Paper Information

Journal: ANALYTICAL AND BIOANALYTICAL CHEMISTRY RESEARCH Year:2018 | Volume:5 | Issue:2 Page(s): 353-361

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Information Journal Paper

Title

Using Boehmite Nanoparticles as an Undercoat, and Riboflavin as a Redox Probe for Immunosensor Designing: Ultrasensitive Detection of Hepatitis C Virus Core Antigen

Author(s)

Valipour Akram | ROUSHANI MAHMOUD | Issue Writer Certificate

Keywords

Boehmite nanoparticles

Hepatitis C virus core antigen

Riboflavin

Immunosensor

Abstract

In this study, a label-free electrochemical Immunosensor for ultrasensitive detection of Hepatitis C virus core antigen in serum samples was fabricated using a simple approach. In this method, a low-cost and sensitive Immunosensor was fabricated based on a Boehmite nanoparticles (BNPs) modified glassy carbon. The BNPs provide a specific platform with an increased surface area which is capable of loading more antibody molecules as a receptor element of Hepatitis C virus core antigen on the electrode surface. It seems that BNP/ antibody conjugate may be an ideal platform for the development of an efficient Immunosensor. It is worth noting that the proposed nanoImmunosensor combines the advantages of the elimination of another substrates in modifying the electrode and reducing the length of the modifying process. The experimental parameters, such as pH and incubation of time were optimized. Under the optimal conditions for modified glassy carbon electrode, a linear relationship was realized in the range of 0. 08-110 pg ml-1, with the detection limit of 10 fg ml-1. The proposed method was applied to the determination of Hepatitis C virus core antigen in serum samples with the desirable results.

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APA: Copy

Valipour, Akram, & ROUSHANI, MAHMOUD. (2018). Using Boehmite Nanoparticles as an Undercoat, and Riboflavin as a Redox Probe for Immunosensor Designing: Ultrasensitive Detection of Hepatitis C Virus Core Antigen. ANALYTICAL AND BIOANALYTICAL CHEMISTRY RESEARCH, 5(2), 353-361. SID. https://sid.ir/paper/354363/en

Vancouver: Copy

Valipour Akram, ROUSHANI MAHMOUD. Using Boehmite Nanoparticles as an Undercoat, and Riboflavin as a Redox Probe for Immunosensor Designing: Ultrasensitive Detection of Hepatitis C Virus Core Antigen. ANALYTICAL AND BIOANALYTICAL CHEMISTRY RESEARCH[Internet]. 2018;5(2):353-361. Available from: https://sid.ir/paper/354363/en

IEEE: Copy

Akram Valipour, and MAHMOUD ROUSHANI, “Using Boehmite Nanoparticles as an Undercoat, and Riboflavin as a Redox Probe for Immunosensor Designing: Ultrasensitive Detection of Hepatitis C Virus Core Antigen,” ANALYTICAL AND BIOANALYTICAL CHEMISTRY RESEARCH, vol. 5, no. 2, pp. 353–361, 2018, [Online]. Available: https://sid.ir/paper/354363/en

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