Trace Analysis of Niflumic Acid in Milk and Human Plasma by Ion-pair-based Vortex Assisted Dispersive Liquid-liquid Microextraction Combined with UV-Vis Spectrophotometry

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Ghoraba Zahra; Aibaghi Behzad; Soleymanpour Ahmad

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Journal: ANALYTICAL AND BIOANALYTICAL CHEMISTRY RESEARCH Year:2018 | Volume:5 | Issue:2 Page(s): 331-342

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Title

Trace Analysis of Niflumic Acid in Milk and Human Plasma by Ion-pair-based Vortex Assisted Dispersive Liquid-liquid Microextraction Combined with UV-Vis Spectrophotometry

Author(s)

Ghoraba Zahra | Aibaghi Behzad | Soleymanpour Ahmad | Issue Writer Certificate

Keywords

Niflumic acid

Vortex-assisted dispersive liquid-liquid microextraction

UV-Vis spectrophotometry

Methylene blue

Milk

Human plasma

Abstract

A simple, accurate and fast Vortex-assisted dispersive liquid-liquid microextraction procedure has been developed for the extractive spectrophotometric determination of Niflumic acid in biological samples. The method is based on the formation of an ion association complex between Niflumic acid and Methylene blue. The resulting ion-pair was extracted into dichloromethane and its absorbance was measured at 655 nm. All experimental parameters affecting the analytical performance of the method such as pH, type and volume of buffer and extraction solvent, dye concentration, extraction time, ionic strength and interfering species were investigated. The calibration curve was linear in the range of 5-70 ng ml-1 and the limit of detection (LOD) was found to be 2. 8 ng ml-1. The procedure was successfully applied for the determination of Niflumic acid in Human plasma and goat’ s Milk samples. The main advantages of the proposed method are rapidity, little solvent consumption, low cost and providing low LOD by the simple UV-Vis spectrophotometer.

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APA: Copy

Ghoraba, Zahra, Aibaghi, Behzad, & Soleymanpour, Ahmad. (2018). Trace Analysis of Niflumic Acid in Milk and Human Plasma by Ion-pair-based Vortex Assisted Dispersive Liquid-liquid Microextraction Combined with UV-Vis Spectrophotometry. ANALYTICAL AND BIOANALYTICAL CHEMISTRY RESEARCH, 5(2), 331-342. SID. https://sid.ir/paper/354368/en

Vancouver: Copy

Ghoraba Zahra, Aibaghi Behzad, Soleymanpour Ahmad. Trace Analysis of Niflumic Acid in Milk and Human Plasma by Ion-pair-based Vortex Assisted Dispersive Liquid-liquid Microextraction Combined with UV-Vis Spectrophotometry. ANALYTICAL AND BIOANALYTICAL CHEMISTRY RESEARCH[Internet]. 2018;5(2):331-342. Available from: https://sid.ir/paper/354368/en

IEEE: Copy

Zahra Ghoraba, Behzad Aibaghi, and Ahmad Soleymanpour, “Trace Analysis of Niflumic Acid in Milk and Human Plasma by Ion-pair-based Vortex Assisted Dispersive Liquid-liquid Microextraction Combined with UV-Vis Spectrophotometry,” ANALYTICAL AND BIOANALYTICAL CHEMISTRY RESEARCH, vol. 5, no. 2, pp. 331–342, 2018, [Online]. Available: https://sid.ir/paper/354368/en

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