IDENTIFICATION OFVERYVIRULENTINFECTIOUS BURSALDISEASE VIRUSES BYRT-PCR OFVP1GENE AND SPECULATION ABOUTTHE POSSIBLE PRESENCE OFREASSORTANTVIRUSES

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GHANIEI A.; PEIGHAMBARI S.M.; RAZMYAR J.

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Journal: Journal of Veterinary Research Year:2011 | Volume:66 | Issue:2 Page(s): 153-159

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Information Journal Paper

Title

IDENTIFICATION OFVERYVIRULENTINFECTIOUS BURSALDISEASE VIRUSES BYRT-PCR OFVP1GENE AND SPECULATION ABOUTTHE POSSIBLE PRESENCE OFREASSORTANTVIRUSES

Author(s)

GHANIEI A. | PEIGHAMBARI S.M. | RAZMYAR J. | Issue Writer Certificate

Keywords

INFECTIOUS BURSAL DISEASE VIRUSQ2

REASSORTANTQ3

VP1Q2

RT-PCRQ2

Abstract

INFECTIOUS BURSAL DISEASE VIRUS (IBDV) genome is a bi-segmented RNAvirus that encodes five viral proteins. The need for the development of a simple and rapid method to differentiate the IBDVs has prompted researchers to work on different viral proteins of these viruses. At the present study, an attempt was made to amplify a 715 bp fragment ofVP1 gene by RT-PCR of 26 IBDV isolates that their virulence pattern had been previously determined by RT-PCR/REA ofVP2 gene. The results showed that in 20 (76%) IBDV isolates, the virulence pattern (very virulent IBDV) were found to be identical using either RT-PCR ofVP1 or RT-PCR/REAof VP2. However, in the remaining six IBDV isolates the results were different. Considering the available farm data from the viruses of this study which are in accordance with results onVP1, it is speculated that this finding may be due to the presence of REASSORTANT IBDVs. These findings also indicate that the VP2in not the sole determinant for the virulence in IBDV.

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APA: Copy

GHANIEI, A., PEIGHAMBARI, S.M., & RAZMYAR, J.. (2011). IDENTIFICATION OFVERYVIRULENTINFECTIOUS BURSALDISEASE VIRUSES BYRT-PCR OFVP1GENE AND SPECULATION ABOUTTHE POSSIBLE PRESENCE OFREASSORTANTVIRUSES. JOURNAL OF VETERINARY RESEARCH, 66(2), 153-159. SID. https://sid.ir/paper/35541/en

Vancouver: Copy

GHANIEI A., PEIGHAMBARI S.M., RAZMYAR J.. IDENTIFICATION OFVERYVIRULENTINFECTIOUS BURSALDISEASE VIRUSES BYRT-PCR OFVP1GENE AND SPECULATION ABOUTTHE POSSIBLE PRESENCE OFREASSORTANTVIRUSES. JOURNAL OF VETERINARY RESEARCH[Internet]. 2011;66(2):153-159. Available from: https://sid.ir/paper/35541/en

IEEE: Copy

A. GHANIEI, S.M. PEIGHAMBARI, and J. RAZMYAR, “IDENTIFICATION OFVERYVIRULENTINFECTIOUS BURSALDISEASE VIRUSES BYRT-PCR OFVP1GENE AND SPECULATION ABOUTTHE POSSIBLE PRESENCE OFREASSORTANTVIRUSES,” JOURNAL OF VETERINARY RESEARCH, vol. 66, no. 2, pp. 153–159, 2011, [Online]. Available: https://sid.ir/paper/35541/en

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