Protective effect of silymarin on viability, motility and mitochondrial membrane potential in spermatozoa treated with alominium

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MOMENI H.R.; SEPEHRI H.; YOSEFI M.; ESKANDARI N.

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Journal: JOURNAL OF CELL & TISSUE Year:2018 | Volume:9 | Issue:2 Page(s): 102-111

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Title

Protective effect of silymarin on viability, motility and mitochondrial membrane potential in spermatozoa treated with alominium

Author(s)

MOMENI H.R. | SEPEHRI H. | YOSEFI M. | ESKANDARI N. | Issue Writer Certificate

Keywords

Aluminum chlorideQ1

ram spermQ1

silymarinQ2

sperm parametersQ1

Abstract

Aim: This study was performed to investigate if silymarin can prevent the adverse effects of Aluminum chloride on viability, motility and mitochondrial membrane potential in ram sperm. Material and Methods: Epididimal spermatozoa from Farahani's ram were divided into five groups: 1. Sperm at 0 hour, 2. Sperm at 180 minutes (control), 3. Sperm treated with Aluminum chloride (0. 5 mM) for 180 minutes, 4. Sperm treated with silymarin (0. 5 μ M) + Aluminum chloride (0. 5 mM) for 180 minutes and 5. Sperm treated with silymarin (0. 5 μ M) for 180 minutes. In order to evaluate viability and mitochondrial membrane potential in the groups, MTT ((3-4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltenyltetrazolium bromide) assay and Rhodamine 123 staining were used respectively. Sperm motility was done according to World Health Organization (WHO) protocol. Data were analysed using one-way ANOVA fallowed by Tukey's test. Results: The percentage of sperm viability, progressive motility and intact mitochondrial membrane potential were significantly decreased in the Aluminum chloride group compared to the control. This pollutant also significantly increased the percentage of non-progressively motile and non-motile sperm compared to the control group. In silymarin + Aluminum chloride group, silymarin could significantly compensate the adverse effect of Aluminum chloride on the sperm parameters (except to non-progressively motile) compared to the Aluminum chloride group. Conclusions: Aluminum chloride has toxic effect on ram sperm viability, motility and intact mitochondrial membrane potential and silymarin is able to compensate the adverse effect of Aluminum chloride on these sperm parameters.

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APA: Copy

MOMENI, H.R., SEPEHRI, H., YOSEFI, M., & ESKANDARI, N.. (2018). Protective effect of silymarin on viability, motility and mitochondrial membrane potential in spermatozoa treated with alominium. JOURNAL OF CELL & TISSUE, 9(2 ), 102-111. SID. https://sid.ir/paper/361406/en

Vancouver: Copy

MOMENI H.R., SEPEHRI H., YOSEFI M., ESKANDARI N.. Protective effect of silymarin on viability, motility and mitochondrial membrane potential in spermatozoa treated with alominium. JOURNAL OF CELL & TISSUE[Internet]. 2018;9(2 ):102-111. Available from: https://sid.ir/paper/361406/en

IEEE: Copy

H.R. MOMENI, H. SEPEHRI, M. YOSEFI, and N. ESKANDARI, “Protective effect of silymarin on viability, motility and mitochondrial membrane potential in spermatozoa treated with alominium,” JOURNAL OF CELL & TISSUE, vol. 9, no. 2 , pp. 102–111, 2018, [Online]. Available: https://sid.ir/paper/361406/en

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