Modulation of MCF7 behavior treated by human adipose stem cells conditioned medium

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Motaghi Moridani F.; HAJI GHASEM KASHANI M.; GHORBANIAN M.T.

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Journal: JOURNAL OF CELL & TISSUE Year:2018 | Volume:9 | Issue:1 Page(s): 12-24

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Title

Modulation of MCF7 behavior treated by human adipose stem cells conditioned medium

Author(s)

Motaghi Moridani F. | HAJI GHASEM KASHANI M. | GHORBANIAN M.T. | Issue Writer Certificate

Keywords

MCF7Q1

Conditioned mediumQ1

ProliferationQ1

CaspaseQ1

Abstract

Aim: In the present study, the anticancer function of CM on MCF7 cells was investigated in vitro. Material and Methods: Adipose stem cells were extracted from cesarean women abdominal fat with their written informed consent at the Velayat hospital in Damghan. CM was prepared from fourth passage of hASCs cultured in serum-free medium for 72h. MCF7 cells were exposed to CM for 24 and 48 hours. Then cell Proliferation rate, survival and apoptotic genes expression were determined using MTT, cell counting (hemocytometer) and RT-PCR. Results: CM-treated MCF7 for 24 and 48h, showed significant decreased of cell Proliferation and viability as compared to the cells cultured in medium containing serum (control). In addition, Caspase3 gene expression of CM-induced cells was increased significantly at 24 and 48h as compared to the control group. While CM-treated cells showed a significant increase of Caspase9 gene expression after 48h induction. It was concluded that CM induced apoptosis by activation of Caspases and reduced Proliferation rate and survival of MCF7 cancer cells. Conclusion: Then Conditioned medium is good candidate to be combined with other anticancer therapies.

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APA: Copy

Motaghi Moridani, F., HAJI GHASEM KASHANI, M., & GHORBANIAN, M.T.. (2018). Modulation of MCF7 behavior treated by human adipose stem cells conditioned medium. JOURNAL OF CELL & TISSUE, 9(1 ), 12-24. SID. https://sid.ir/paper/361584/en

Vancouver: Copy

Motaghi Moridani F., HAJI GHASEM KASHANI M., GHORBANIAN M.T.. Modulation of MCF7 behavior treated by human adipose stem cells conditioned medium. JOURNAL OF CELL & TISSUE[Internet]. 2018;9(1 ):12-24. Available from: https://sid.ir/paper/361584/en

IEEE: Copy

F. Motaghi Moridani, M. HAJI GHASEM KASHANI, and M.T. GHORBANIAN, “Modulation of MCF7 behavior treated by human adipose stem cells conditioned medium,” JOURNAL OF CELL & TISSUE, vol. 9, no. 1 , pp. 12–24, 2018, [Online]. Available: https://sid.ir/paper/361584/en

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