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Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Issue Info: 
  • Year: 

    2018
  • Volume: 

    9
  • Issue: 

    1
  • Pages: 

    1-11
Measures: 
  • Citations: 

    0
  • Views: 

    399
  • Downloads: 

    0
Abstract: 

Aim: The current research was done to investigate the sperm plasma membrane integrity, oxidative stress factors of sperm and testis in male mice treated with cadmium. Material and Methods: 24 adult NMRI mice were divided into four groups: 1. Control group 2. Treated with cadmium chloride (5 mg/kg) 3. Treated with Curcumin (100 mg/kg) 4. Treated with curcumin + cadmium chloride. The treatments were performed as a single dose and after 24 hours, epididymal spermatozoa of different groups were used to evaluate the plasma membrane integrity. In addition, the amount of malondialdehyde (MDA) and the anti-oxidant activity of serum and testis were evaluated. Data were analyzed using ANOVA test followed by Turkey’ s test (p<0. 05). Results: findings showed that cadmium chloride caused a significant decrement in plasma membrane integrity compared to the control groups. In addition, cadmium chloride induced a significant increment of MDA in serum and testis and a significant decrement in total antioxidant activity of serum and testis as compared to the group. In curcumin + cadmium chloride-treated group, curcumin could significantly compensate the toxic effect of cadmium on these parameters compared to the cadmium-treated group. Conclusion: it seems that curcumin as a potent antioxidant is able to ameliorate the adverse effects of cadmium chloride on sperm plasma membrane integrity, lipid oxidation and total antioxidant activity of serum and testis.

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Issue Info: 
  • Year: 

    2018
  • Volume: 

    9
  • Issue: 

    1
  • Pages: 

    12-24
Measures: 
  • Citations: 

    0
  • Views: 

    342
  • Downloads: 

    0
Abstract: 

Aim: In the present study, the anticancer function of CM on MCF7 cells was investigated in vitro. Material and Methods: Adipose stem cells were extracted from cesarean women abdominal fat with their written informed consent at the Velayat hospital in Damghan. CM was prepared from fourth passage of hASCs cultured in serum-free medium for 72h. MCF7 cells were exposed to CM for 24 and 48 hours. Then cell proliferation rate, survival and apoptotic genes expression were determined using MTT, cell counting (hemocytometer) and RT-PCR. Results: CM-treated MCF7 for 24 and 48h, showed significant decreased of cell proliferation and viability as compared to the cells cultured in medium containing serum (control). In addition, caspase3 gene expression of CM-induced cells was increased significantly at 24 and 48h as compared to the control group. While CM-treated cells showed a significant increase of caspase9 gene expression after 48h induction. It was concluded that CM induced apoptosis by activation of caspases and reduced proliferation rate and survival of MCF7 cancer cells. Conclusion: Then conditioned medium is good candidate to be combined with other anticancer therapies.

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Author(s): 

RAHNAMA H. | Helali L. | AHMADI N.

Issue Info: 
  • Year: 

    2018
  • Volume: 

    9
  • Issue: 

    1
  • Pages: 

    25-34
Measures: 
  • Citations: 

    0
  • Views: 

    277
  • Downloads: 

    0
Abstract: 

Aim: In order to study the efficiency of the tuber specific promoter (Patatin1), the expression of the HBsAg antigen of the hepatitis B vaccine was evaluated using a transient expression method (AgroInfiltration) in the tubers and leaves of potato plants. Material and Methods: A tuber specific promoter (Pat1) was isolated from the potato plant using a specific primer pairs by Polymerase Chain Reaction (PCR). It was cloned in the upstream of a synthetic optimized codon of the HBsAg gene in the binary plant vector pBI121. In order to compare the tissue specificity of Pat1, the HBsAg gene also was used under the control of a consultative CaMV35S promoter. The genetic constructs were transferred to the tubers and leaves of potato plants using the Agroinfiltration method. An HBsAg antigen content was measured using ELISA method. Results: The level of HBsAg antigens in the leaves and tubers of potato plants indicated that Pat1 promoter specifically induced HBsAg high expression in the tuber tissues. Very low expression by the Pat1 promoter in the leaf tissues has been also reported and can be partly dependent on the presence of inducing factors in the inoculation media. However, the expression of HBsAg antigen occurs in both leaf and tuber tissues under the consultative promoter CaMV35S. The results showed that the codon optimized HBsAg gene for potato plant was expressed properly in plant tissues. Conclusion: findings indicate that potato tuber specific promoter Pat1 can be used effectively to express the synthetic optimized HBsAg antigen in the transgenic potato plants.

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Author(s): 

GHOLAMI A.A. | TARINEJAD A.

Issue Info: 
  • Year: 

    2018
  • Volume: 

    9
  • Issue: 

    1
  • Pages: 

    35-55
Measures: 
  • Citations: 

    0
  • Views: 

    740
  • Downloads: 

    0
Abstract: 

Aim: In the current study, the effect of growth regulators, type and composition of the culture medium, genotypes of the used cultivars and type of explants were investigated on the ability of callusing and regeneration of wheat cultivars. Material and methods: two culture media (N6, MS), three immature embryos, mature embryos and leaf cuttings were used. N6 medium containing growth regulator 2, 4-D was used for callusing of embryo and the leaf pieces, but for regeneration N6 medium containing growth regulators NAA, BAP and Kin was used. For calligraphy and regeneration of immature embryos, MS medium containing a variety of growth regulators were used. Results: Callus induction and regeneration differed based on the genotype, type of explants and media composition. Chamran variety had the highest rate of callus and regeneration from immature embryo. For the adult embryo, the highest level of calligenesis and regeneration related to the C-D-9 line. In the case of leaf parts, the highest level of callus related to the C-D-9 at the level of 2, 4-D (2. 4mg/L). The highest percentage of shoots belonged to the C-D-9 line that was obtained in N6 medium (6) containing IAA (1mg/L) + BA (1mg/L). Conclusion: Response to tissue culture in wheat is affected by several factors such as genotype, type of explants, growth regulators. Results proved that the type and concentration of used growth regulators in the culture medium differ among cultivars for callus induction and wheat regeneration. The most important factor affecting wheat cultivation was immature embryo explant and the genotype type of the used cultivar.

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Issue Info: 
  • Year: 

    2018
  • Volume: 

    9
  • Issue: 

    1
  • Pages: 

    56-65
Measures: 
  • Citations: 

    0
  • Views: 

    462
  • Downloads: 

    0
Abstract: 

Aim: The current study was designed to understand the destructive effects of mancozeb on BTB in an in vitro model. Material and Methods: Male mice were gavage-administered MZB (250, 500 mg/kg w. w) for 40 consecutive days. Integrity of BTB was evaluated using Evans blue dye. The relative mRNA expression of BTB-associated genes; including N-cadherin, claudin-11 and zonula occludens-1 was investigated by quantitative real-time reverse transcription– polymerase chain reaction in different groups (qRT-PCR). Results: Concentration of Evans Blue dye in the lumen of seminiferous tubules was increased in mancozeb-treated animals. The mRNA expression levels of N-cadherin, claudin-11 and zonula occludens-1 genes were decreased significantly in the MZB 500 mg/kg group compared with the control group. Conclusion: According to the obtained data, it could be concluded that mancozeb increase BTB permeability leading to testicular dysfunction by the alteration of BTB-associated genes.

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Issue Info: 
  • Year: 

    2018
  • Volume: 

    9
  • Issue: 

    1
  • Pages: 

    66-75
Measures: 
  • Citations: 

    0
  • Views: 

    436
  • Downloads: 

    0
Abstract: 

Aim: The aims of study were to investigate callogenesis and regeneration of the studied cultivars and also exploring the possibility of gene transfer into maize by using complete bud and stem apical meristem explants. Material and methods: Complete bud and stem apical meristem of six cultivars were used as explants on MS medium + 5 mg. L-¹ 2, 4-D. For regeneration, calluses were cultured in MS including 1 mg. L-¹ Kn and 10 mg. L-¹ BAP. According to the results of tissue culture, SC703 and SC704 cultivars were selected for gene transfer. Transformation of explants was done with LBA4404 strain of Agrobacterium tumefaciens containing pBI121 vector. To investigate the transgenic nature of the samples, PCR and histochemical assay were used. Results: Callus induction was taking place after 30 to 40 days and the frequency of callus induction in six cultivars was 100%, in addition the highest rate of regeneration (90 %) was observed in stem apical meristem explants of SC704. In some cultivars, stem apical meristem explants were more suitable, although in other cultivars, complete bud explants were better. PCR results for NPTII gene and GUS histochemical assay showed that some calli are transgenic and GUS gene is expressed. Conclusion: The results of callogenesis, regeneration and genetic transformation showed that SC 704 was better than the others.

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Issue Info: 
  • Year: 

    2018
  • Volume: 

    9
  • Issue: 

    1
  • Pages: 

    76-85
Measures: 
  • Citations: 

    0
  • Views: 

    366
  • Downloads: 

    0
Abstract: 

Aim: The aim of this study was to evaluate the effect of silymarin and lithium chloride on DNA integrity and nucleus of ram sperm. Material and Methods: In this study, Farahani's ram testes were obtained from Arak slaughterhouse immediately after ram daily slaughter and transferred to the research laboratory. A few incisions were made in the epididymis, and spermatozoa were then washed into a sterile falcon tube by Ham's F10 medium. collected spermatozoa of ram were divided into four groups: 1. Sperm at 0 hour, 2. Sperm incubated for180 minutes (control), 3. Sperm treated with lithium chloride for 180 minutes and 4. Sperm treated with silymarin + lithium chloride for 180 minutes. DNA integrity and DNA fragmentation were investigated by acridine orange staining sperm chromatin expersion (SCD) test respectively. Morphological feature of apoptosis in sperm nucleus was assessed using Diff-Quick staining. Data were analyzed using one-way analysis of variance test (ANOVA) followed by Turkey's test. Results: The percentage of DNA fragment and apoptosis were significantly increased in lithium chloride-treated group compared to the control. In silymarin+ lithium chloride group, Silymarin could signicantly compensate these effect compared to the lithium choloride group. Conclusion: Silymarin as a potent antioxidant could prevent toxic effect of lithium on DNA fragmentation and apoptosis in sperm nucleus.

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Author(s): 

GHASEMI S. | EHSANPOUR A.A.

Issue Info: 
  • Year: 

    2018
  • Volume: 

    9
  • Issue: 

    1
  • Pages: 

    86-101
Measures: 
  • Citations: 

    0
  • Views: 

    433
  • Downloads: 

    0
Abstract: 

Aim: In this study, the effect of IAA on salt tolerance of tobacco plants (Nicotiana plumbaginifolia) was investigated under in vitro culture conditions. Material and Methods: Sterile seeds of N. plumbaginifolia were cultured in MS medium. In vitro propagated seedlings under tissue culture conditions were treated with IAA (mg / l) and salinity (0, 100 and 200 mM) for 4 weeks. Physiological and biochemical parameters including H2O2, chlorophyll, soluble carbohydrate, protein and antioxidant enzyme activity were measured. Results: Salt stress increased H2O2, proline, catalase, superoxide dismutase and ascorbate peroxidase, while reverse patterns were observed for photosynthetic pigments, soluble carbohydrates and also total protein. In salt-treated seedlings, application of auxin increased proline, catalase and H2O2, but decreased photosynthetic pigmentation, soluble carbohydrates, total protein and some enzymes such as superoxide dismutase and ascorbate peroxidase. Conclusion: Auxin treatment in salt-treated tobacco seedlings improved salinity tolerance by changes in some physiological parameters.

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