Optimization of corn steep liquor utilization for recombinant endoglucanase II enzyme production by methylotrophic yeast

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Jafari manesh Seyed Mojtaba; RASHEDI HAMID; ARJMAND SAREH; RANAEI SIADAT SEYED OMID

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Journal: JOURNAL OF BIOTECHNOLOGY Year:2017 | Volume:8 | Issue:3 Page(s): 0-0

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Title

Optimization of corn steep liquor utilization for recombinant endoglucanase II enzyme production by methylotrophic yeast

Author(s)

Jafari manesh Seyed Mojtaba | RASHEDI HAMID | ARJMAND SAREH | RANAEI SIADAT SEYED OMID | Issue Writer Certificate

Keywords

optimizationQ2

endoglucanaseIIQ1

corn steep liquorQ1

methylotrophic yeastQ1

response surface methodologyQ1

Abstract

Cellulase is one of the industrial enzymes which its production and utilization is increasingly taking into consideration due to global heed to second-generation bioethanol production. Cellulase produced by different organisms such as fungi, bacteria, insects, and plants. With increase in utilization of this enzyme and need for reduction in the enzymes price for production of second-generation bioethanol, the production of recombinant enzyme has been considered noticeably. In this study, by investigation of corn steep liquor as nitrogen source and second carbon source after glycerol, a new medium is designed based on SYN6 salt medium then biomass and endoglucanase II production by methylotrophic yeast was optimized. Experiments designed by one-factor and response surface methodology used for optimization. Results showed that optimum conditions for biomass and endoglucanase production are 5. 5% (w/v) and 6. 15% (w/v) of corn steep liquor respectively. New optimized conditions increased 41. 4% and 69. 7% for biomass and recombinant enzyme production respectively.

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APA: Copy

Jafari manesh, Seyed Mojtaba, RASHEDI, HAMID, ARJMAND, SAREH, & RANAEI SIADAT, SEYED OMID. (2017). Optimization of corn steep liquor utilization for recombinant endoglucanase II enzyme production by methylotrophic yeast. JOURNAL OF BIOTECHNOLOGY, 8(3 ), 0-0. SID. https://sid.ir/paper/362972/en

Vancouver: Copy

Jafari manesh Seyed Mojtaba, RASHEDI HAMID, ARJMAND SAREH, RANAEI SIADAT SEYED OMID. Optimization of corn steep liquor utilization for recombinant endoglucanase II enzyme production by methylotrophic yeast. JOURNAL OF BIOTECHNOLOGY[Internet]. 2017;8(3 ):0-0. Available from: https://sid.ir/paper/362972/en

IEEE: Copy

Seyed Mojtaba Jafari manesh, HAMID RASHEDI, SAREH ARJMAND, and SEYED OMID RANAEI SIADAT, “Optimization of corn steep liquor utilization for recombinant endoglucanase II enzyme production by methylotrophic yeast,” JOURNAL OF BIOTECHNOLOGY, vol. 8, no. 3 , pp. 0–0, 2017, [Online]. Available: https://sid.ir/paper/362972/en

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