Single Nucleotide Polymorphism in part of GH Gene and Its Association with Carcass Traits in Afshari and Afshari-Booroola Merino sheep

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SEPEHRI R.; HARKINEZHAD T.; ALIJANI S.; Shoja Ghias j.; RAAFAT S.A.

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Journal: Journal of Molecular and Cellular Researches Year:2018 | Volume:31 | Issue:2 Page(s): 188-202

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Title

Single Nucleotide Polymorphism in part of GH Gene and Its Association with Carcass Traits in Afshari and Afshari-Booroola Merino sheep

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Keywords

Afshri sheepQ1

growth hormonQ1

multiple birthsQ1

carcass qualityQ2

SNPQ1

Abstract

growth hormone is one of the main factors affecting the growth of animals. Dِِifferent alleles of this gene, may lead to different phenotypic effects. The aim of this study was to identify alleles of this gene in exon 5 and part of the 3′ downstream area. For this purpose, 133 lambs from a flock and ultimately at the same age in three groups of Afshari pure breed and F2 and F5 crosses of Afshari-Booroola Merino were used. After taking Blood samples and recording the phenotypic measurements on live animals, 85 lambs were slaughtered. Afterward, carcass weight was measured. After 24 hours storing the carcasses in the cold room the weight of thigh, shoulder, neck, fat tail right side of carcass and waste was measured. Regression coefficient analyses were used to estimate carcass traits of those animals that were not slaughtered. DNA was extracted by phenol-chloroform procedures from blood samples. Then, using designed primers, the desired fragment was amplified and directly sequenced. Afterward, by analyzing of sequences, association of genotypes with carcass traits were evaluated. The results showed that in the nucleotide site of 2846 there were two genotypes, TT and TC, affecting the length of the body in Afshari pure breed and F5 crosses. In position 2882 sequence of the gene, a nucleotide was inserted in some animals and this insertion only in F2 crosses was associated with waste (carcass fat). Our results on the observed variations in case be proved by further evaluations, it can be used in marker assisted selection program in sheep breeding.

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APA: Copy

SEPEHRI, R., HARKINEZHAD, T., ALIJANI, S., Shoja Ghias, j., & RAAFAT, S.A.. (2018). Single Nucleotide Polymorphism in part of GH Gene and Its Association with Carcass Traits in Afshari and Afshari-Booroola Merino sheep. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 31(2 ), 188-202. SID. https://sid.ir/paper/366884/en

Vancouver: Copy

SEPEHRI R., HARKINEZHAD T., ALIJANI S., Shoja Ghias j., RAAFAT S.A.. Single Nucleotide Polymorphism in part of GH Gene and Its Association with Carcass Traits in Afshari and Afshari-Booroola Merino sheep. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2018;31(2 ):188-202. Available from: https://sid.ir/paper/366884/en

IEEE: Copy

R. SEPEHRI, T. HARKINEZHAD, S. ALIJANI, j. Shoja Ghias, and S.A. RAAFAT, “Single Nucleotide Polymorphism in part of GH Gene and Its Association with Carcass Traits in Afshari and Afshari-Booroola Merino sheep,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 31, no. 2 , pp. 188–202, 2018, [Online]. Available: https://sid.ir/paper/366884/en

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