The effects of plant growth regulators and explants on callus induction in Ajowan

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FAZELI NASAB B.; Fooladvand Z.

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Journal: Journal of Molecular and Cellular Researches Year:2019 | Volume:32 | Issue:1 Page(s): 63-75

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Title

The effects of plant growth regulators and explants on callus induction in Ajowan

Author(s)

FAZELI NASAB B. | Fooladvand Z. | Issue Writer Certificate

Keywords

AjowanQ1

CallusQ1

2Q2

4-DQ2

BAPQ1

Abstract

Ajowan (Carum copticum) has been considered as an important medicinal plant because it contains many alkaloids such as Thymol. In vitro culture of Ajowan provides new tissue sources such as Callus, cell suspension and seedlings to produce secondary metabolites. The present study describes Callus production optimization procedures experiment that was a factorial experiment based on completely randomized design at three levels with four explants (root, shoot, leaf and coleoptile) on Murashige and Skoog (MS) medium supplemented with different concentrations of BAP (0. 25, 0. 5 and 1 mg/l) and 2, 4-D(2, 4 and 8 mg/l). Comparison of means showed that the maximum Callus production was obtained from shoot explants, the coleoptile and leaf explants were in the second orders. In overall, 0. 25 mg /l BAP with 2 mg 2, 4-D mg/l concentrations proved to be optimal for the production of maximum Callus induction.

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APA: Copy

FAZELI NASAB, B., & Fooladvand, Z.. (2019). The effects of plant growth regulators and explants on callus induction in Ajowan. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 32(1 ), 63-75. SID. https://sid.ir/paper/369666/en

Vancouver: Copy

FAZELI NASAB B., Fooladvand Z.. The effects of plant growth regulators and explants on callus induction in Ajowan. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2019;32(1 ):63-75. Available from: https://sid.ir/paper/369666/en

IEEE: Copy

B. FAZELI NASAB, and Z. Fooladvand, “The effects of plant growth regulators and explants on callus induction in Ajowan,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 32, no. 1 , pp. 63–75, 2019, [Online]. Available: https://sid.ir/paper/369666/en

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