Effect of salinity stress on the gene expression of Na/K ATPase on two Artemia populations (Artemia franciscana and Parthenogenetic Artemia) by RT-PCR-DGGE

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MOHAMMADZADEH M.; YOUSEFI K.; NAJDE GERAMI E.H.

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Journal: Journal of Animal Research Year:2019 | Volume:31 | Issue:4 Page(s): 459-471

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Title

Effect of salinity stress on the gene expression of Na/K ATPase on two Artemia populations (Artemia franciscana and Parthenogenetic Artemia) by RT-PCR-DGGE

Author(s)

MOHAMMADZADEH M. | YOUSEFI K. | NAJDE GERAMI E.H. | Issue Writer Certificate

Keywords

ArtemiaQ1

salinity stressQ1

sodium-potassium pumpQ1

RT-PCRQ2

DGGE techniqueQ1

Abstract

Artemia is One of types of crustacean that can withstand in very high salinity. Due to the climate changes and increasing the salinity of inland waters and importance of Artemia as a main food sources for crustacean and marine fish larvae as well, it is necessary to understand the molecular mechanisms of biological processes in different populations of Artemia. The current study, investigates the effects of salinity stress on Na + / K ATPase gene expression in exon 7 of two Artemia population with using of RT-RCR-DGGE. Two different populations of Artemia franciscana and Parthenogenetic Artemia were reared at salinities 60, 120 and 180 mg/l. At the end of the experiment the mRNA of Na + / K ATPase was provided and converted to cDNA. Subsequent DGGE and sequencing analyses revealed that the products of these genes in A. franciscana is homozygous while in Parthenogenetic Artemia is heterozygous which is produced by two alleles. Also it was found that in the Parthenogenetic Artemia protein translation and the production of two different alleles is obtained. Interestingly, no difference was observed in the samples studied and apparently the only difference can be related to genomic variation among these populations.

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APA: Copy

MOHAMMADZADEH, M., YOUSEFI, K., & NAJDE GERAMI, E.H.. (2019). Effect of salinity stress on the gene expression of Na/K ATPase on two Artemia populations (Artemia franciscana and Parthenogenetic Artemia) by RT-PCR-DGGE. JOURNAL OF ANIMAL RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 31(4 ), 459-471. SID. https://sid.ir/paper/369944/en

Vancouver: Copy

MOHAMMADZADEH M., YOUSEFI K., NAJDE GERAMI E.H.. Effect of salinity stress on the gene expression of Na/K ATPase on two Artemia populations (Artemia franciscana and Parthenogenetic Artemia) by RT-PCR-DGGE. JOURNAL OF ANIMAL RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2019;31(4 ):459-471. Available from: https://sid.ir/paper/369944/en

IEEE: Copy

M. MOHAMMADZADEH, K. YOUSEFI, and E.H. NAJDE GERAMI, “Effect of salinity stress on the gene expression of Na/K ATPase on two Artemia populations (Artemia franciscana and Parthenogenetic Artemia) by RT-PCR-DGGE,” JOURNAL OF ANIMAL RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 31, no. 4 , pp. 459–471, 2019, [Online]. Available: https://sid.ir/paper/369944/en

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