ITS-rDNA and molecular typing of Leishmania spp. in suspected patients with cutaneous leishmaniasis in Sistan and Balochestan province, Iran

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ZARE ZADEH A.; Motlleb Gh.R.; MIRAHMADI H.; SALIMI KHORASHAD A.R.

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Journal: Journal of Molecular and Cellular Researches Year:2019 | Volume:32 | Issue:1 Page(s): 125-135

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Title

ITS-rDNA and molecular typing of Leishmania spp. in suspected patients with cutaneous leishmaniasis in Sistan and Balochestan province, Iran

Author(s)

ZARE ZADEH A. | Motlleb Gh.R. | MIRAHMADI H. | SALIMI KHORASHAD A.R. | Issue Writer Certificate

Keywords

ITS-rDNAQ1

Leishmania majorQ2

Leishmania tropicaQ1

PCR-RFLPQ1

Abstract

Leishmaniasis is a parasitic disease caused by Leishmania species. The early diagnosis focused on clinical symptoms and direct observation of the parasites. Molecular methods are more sensitive than the direct microscopy. The identity of the species in Sistan-Baluchistan province has not been taken yet. ITS-rDNA was used to detect the species of Leishmania in patients from Sistan-Baluchistan province using molecular methods. This study was conducted during 2014-2015. Positive smear samples (82) were collected for molecular studies. The parasites were inoculated in N. N. N culture (with RPMI-1640 medium and 10% fetal calf serum) for rapid proliferation. After DNA extraction, the PCR-RFLP was carried out to determine the Leishmania species. SPSS and Multalin software were used to analyse the results. Forty six (56%) and 36 patients (44%) were diagnosed Leishmania major and Tropica respectively. The dominant species in the city of Chabahar and Mirjaveh were Leishmania tropica and Leishmania major respectively. In the central region of the province, both Leishmania major and tropica are responsible for the disease. The results of this study indicated that both forms of L. major and L. tropica were identified in Sistan and Balochestan Province in Iran, which could be due to unlawful entry from Pakistan and Afghanistan. PCR-RFLP has high sensitivity for the diagnosis of leishmaniasis and rapid species identification of the parasites.

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APA: Copy

ZARE ZADEH, A., Motlleb, Gh.R., MIRAHMADI, H., & SALIMI KHORASHAD, A.R.. (2019). ITS-rDNA and molecular typing of Leishmania spp. in suspected patients with cutaneous leishmaniasis in Sistan and Balochestan province, Iran. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 32(1 ), 125-135. SID. https://sid.ir/paper/371221/en

Vancouver: Copy

ZARE ZADEH A., Motlleb Gh.R., MIRAHMADI H., SALIMI KHORASHAD A.R.. ITS-rDNA and molecular typing of Leishmania spp. in suspected patients with cutaneous leishmaniasis in Sistan and Balochestan province, Iran. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2019;32(1 ):125-135. Available from: https://sid.ir/paper/371221/en

IEEE: Copy

A. ZARE ZADEH, Gh.R. Motlleb, H. MIRAHMADI, and A.R. SALIMI KHORASHAD, “ITS-rDNA and molecular typing of Leishmania spp. in suspected patients with cutaneous leishmaniasis in Sistan and Balochestan province, Iran,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 32, no. 1 , pp. 125–135, 2019, [Online]. Available: https://sid.ir/paper/371221/en

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