Isolation, identification and study of defensin gene structure (Def1) in Raphanus sativus

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ETEBARI M.; MOTALLEBI M.; ZAMANI M.R.; MOGHADDASSI JAHROMI Z.

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Journal: Journal of Molecular and Cellular Researches Year:2019 | Volume:32 | Issue:1 Page(s): 136-145

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Title

Isolation, identification and study of defensin gene structure (Def1) in Raphanus sativus

Author(s)

ETEBARI M. | MOTALLEBI M. | ZAMANI M.R. | MOGHADDASSI JAHROMI Z. | Issue Writer Certificate

Keywords

Raphanus sativusQ1

DefensinQ1

Def1 geneQ1

gene isolationQ1

cloningQ1

Abstract

Different studies have demonstrated that the plants, by producing antifungal peptides, such as defencins, cause the pores in the fungal plasma membrane which leads to efflux and influx of cellular components. These changes ultimately lead to fungal cell death. The purpose of this research was to isolate, identify, and study of defencin gene structure in Raphanus sativus by cloning and sequencing. The genomic DNA extraction from Raphanus sativus was achieved by CTAB method. Amplification of this gene was performed using the specific primers (RDeff /RDefr). The amplified DNA fragment (356bp) was cloned into pJET1. 2 cloning vector and the constructs were confirmed via enzyme digestion and PCR patterns. Also, the cDNA of this gene, which was about 243bp, was synthesized after extraction of RNA by specific primers. The amplified fragment of the cDNA was confirmed, cloned in the pJET1. 2 vector and sequenced. Comparison of genomic DNA and cDNA sequences showed that Def1gene contains one intron (113bp) and its open reading frame encodes a peptide with a length of 80 amino acids. Alignment of the amino acid sequence of Def1 shows 90 to 98. 8% homology with other reported Defensin sequences. The identification and cloning of this gene could be used in future for fungal diseases management.

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APA: Copy

ETEBARI, M., MOTALLEBI, M., ZAMANI, M.R., & MOGHADDASSI JAHROMI, Z.. (2019). Isolation, identification and study of defensin gene structure (Def1) in Raphanus sativus. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 32(1 ), 136-145. SID. https://sid.ir/paper/371222/en

Vancouver: Copy

ETEBARI M., MOTALLEBI M., ZAMANI M.R., MOGHADDASSI JAHROMI Z.. Isolation, identification and study of defensin gene structure (Def1) in Raphanus sativus. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2019;32(1 ):136-145. Available from: https://sid.ir/paper/371222/en

IEEE: Copy

M. ETEBARI, M. MOTALLEBI, M.R. ZAMANI, and Z. MOGHADDASSI JAHROMI, “Isolation, identification and study of defensin gene structure (Def1) in Raphanus sativus,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 32, no. 1 , pp. 136–145, 2019, [Online]. Available: https://sid.ir/paper/371222/en

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