Application of a High-throughput Polyacrylamide Electrophoresis Gel System with Ethidium Bromide Staining in Genetic Diversity Estimation of Sunflower Genotypes Using TRAP Markers

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ZEINALZADEH TABRIZI HOSSEIN; Hosseinpour Arash

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Agricultural Biotechnology Year:2018 | Volume:17 | Issue:1 Page(s): 69-79

Download Full-Text

Persian Verion

View:

335

Download:

Cites:

Information Journal Paper

Title

Application of a High-throughput Polyacrylamide Electrophoresis Gel System with Ethidium Bromide Staining in Genetic Diversity Estimation of Sunflower Genotypes Using TRAP Markers

Author(s)

ZEINALZADEH TABRIZI HOSSEIN | Hosseinpour Arash | Issue Writer Certificate

Keywords

SunflowerQ1

Genetic diversityQ2

PolymorphismQ2

ElectrophoresisQ3

Abstract

In this research, the application of new Electrophoresis method of polyacrylamide gel using ethidium bromide staining for genetic variation of Sunflower genotypes using TRAP novel marker has been proposed which has advantages such as low-cost, higher speed of analysis, analysis of a large number of samples per unit time (196×2) per experiment, as well as the reuse of gels in comparison with conventional methods of silver staining and fluorescent primer labeling. Six fixed and six arbitrary primers produced 19 TRAP markers so that from 116 bands, 109 of them were polymorphic. Mean number of polymorphic bands were 5. 79. DNA fragments size were between 25-920 bp. Polymorphic Information Content (PIC) of this marker were between 0. 03 and 0. 50 (with the average of 0. 33). Primer combination (MAX3BR and Sa12) with PIC value of 0. 49 was the highest polymorphic locus. The results showed that this method could be very useful not only in detecting products of the microsatellite markers, but also in other markers, such as TRAP, which produces many polymorphic bands.

Cites

No record.

References

No record.

Cite

APA: Copy

ZEINALZADEH TABRIZI, HOSSEIN, & Hosseinpour, Arash. (2018). Application of a High-throughput Polyacrylamide Electrophoresis Gel System with Ethidium Bromide Staining in Genetic Diversity Estimation of Sunflower Genotypes Using TRAP Markers. AGRICULTURAL BIOTECHNOLOGY (AGRICULTURAL RESEARCH), 17(1 ), 69-79. SID. https://sid.ir/paper/373962/en

Vancouver: Copy

ZEINALZADEH TABRIZI HOSSEIN, Hosseinpour Arash. Application of a High-throughput Polyacrylamide Electrophoresis Gel System with Ethidium Bromide Staining in Genetic Diversity Estimation of Sunflower Genotypes Using TRAP Markers. AGRICULTURAL BIOTECHNOLOGY (AGRICULTURAL RESEARCH)[Internet]. 2018;17(1 ):69-79. Available from: https://sid.ir/paper/373962/en

IEEE: Copy

HOSSEIN ZEINALZADEH TABRIZI, and Arash Hosseinpour, “Application of a High-throughput Polyacrylamide Electrophoresis Gel System with Ethidium Bromide Staining in Genetic Diversity Estimation of Sunflower Genotypes Using TRAP Markers,” AGRICULTURAL BIOTECHNOLOGY (AGRICULTURAL RESEARCH), vol. 17, no. 1 , pp. 69–79, 2018, [Online]. Available: https://sid.ir/paper/373962/en

Related Journal Papers