Improvement of culture media and PGRs in Pistacia vera cv. Ghazvini micropropagation

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GAROOSI GH.A.; MALEKI S.; Nezami Alanagh E.

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Journal: Journal of Plant Research Year:2018 | Volume:31 | Issue:2 Page(s): 383-395

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Title

Improvement of culture media and PGRs in Pistacia vera cv. Ghazvini micropropagation

Author(s)

GAROOSI GH.A. | MALEKI S. | Nezami Alanagh E. | Issue Writer Certificate

Keywords

Pistacia veraQ2

Ghazvini rootstockQ1

micropropagationQ2

L-gluthaminQ1

plant growth regulators (PGRs)Q1

Abstract

Pistacia vera cv. Ghazvini rootstock is considered as one of the important pistachio rootstocks in tolerance to salinity, calcareous and resistant to drought. In order to providing a reliable protocol for micro-propagation of this rootstock, the efficiency of modified GNH media (GNH-A and GNH-B) compared to MS, WPM, DKW or GNH media was studied. Moreover, the effect of different concentration of BAP (1. 1, 1. 8 or 3. 6 mg/l) and L-glutamine (0. 0, 400, 800 or 1600 mg/l) to improve shoot-let production rate with the lowest physiological disorders was survived. Results revealed that the greatest buds and shoots number per explants (34. 46 ± 3. 16 and 4. 23 ± 0. 46, respectively) were attained significantly on GNH-A medium compared to the other media studied. Integration of BAP at different concentrations, affected significantly all the studied growth traits, producing the highest shoot number (6. 66 ± 0. 48) when GNHA was supplemented with 3. 6 mg/l BAP. Furthermore, application of exogenous 800 mg/l L-glutamine in GNH-A medium improved significantly both shoots height and fresh weight (1. 20± 0. 06cm and 0. 31± 0. 02g, respectively), in comparison with control. In rooting stage, the incorporation of NAA into media significantly increased rooting percentage compared to IBA. Finally, we strongly believe that these results will open new insights for researchers towards efficient culture media improvements for economically important woody plants, Pistacia vera species, in particular.

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APA: Copy

GAROOSI, GH.A., MALEKI, S., & Nezami Alanagh, E.. (2018). Improvement of culture media and PGRs in Pistacia vera cv. Ghazvini micropropagation. JOURNAL OF PLANT RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 31(2 ), 383-395. SID. https://sid.ir/paper/395954/en

Vancouver: Copy

GAROOSI GH.A., MALEKI S., Nezami Alanagh E.. Improvement of culture media and PGRs in Pistacia vera cv. Ghazvini micropropagation. JOURNAL OF PLANT RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2018;31(2 ):383-395. Available from: https://sid.ir/paper/395954/en

IEEE: Copy

GH.A. GAROOSI, S. MALEKI, and E. Nezami Alanagh, “Improvement of culture media and PGRs in Pistacia vera cv. Ghazvini micropropagation,” JOURNAL OF PLANT RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 31, no. 2 , pp. 383–395, 2018, [Online]. Available: https://sid.ir/paper/395954/en

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