The effect of IAA on stress resistance of tobacco seedlings (Nicotiana plumbaginifolia) under in vitro culture conditions

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GHASEMI S.; EHSANPOUR A.A.

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Paper Information

Journal: JOURNAL OF CELL & TISSUE Year:2018 | Volume:9 | Issue:1 Page(s): 86-101

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Title

The effect of IAA on stress resistance of tobacco seedlings (Nicotiana plumbaginifolia) under in vitro culture conditions

Author(s)

GHASEMI S. | EHSANPOUR A.A. | Issue Writer Certificate

Keywords

AuxinQ2

Salinity stressQ2

TobaccoQ1

Abstract

Aim: In this study, the effect of IAA on salt tolerance of Tobacco plants (Nicotiana plumbaginifolia) was investigated under in vitro culture conditions. Material and Methods: Sterile seeds of N. plumbaginifolia were cultured in MS medium. In vitro propagated seedlings under tissue culture conditions were treated with IAA (mg / l) and salinity (0, 100 and 200 mM) for 4 weeks. Physiological and biochemical parameters including H2O2, chlorophyll, soluble carbohydrate, protein and antioxidant enzyme activity were measured. Results: Salt stress increased H2O2, proline, catalase, superoxide dismutase and ascorbate peroxidase, while reverse patterns were observed for photosynthetic pigments, soluble carbohydrates and also total protein. In salt-treated seedlings, application of Auxin increased proline, catalase and H2O2, but decreased photosynthetic pigmentation, soluble carbohydrates, total protein and some enzymes such as superoxide dismutase and ascorbate peroxidase. Conclusion: Auxin treatment in salt-treated Tobacco seedlings improved salinity tolerance by changes in some physiological parameters.

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APA: Copy

GHASEMI, S., & EHSANPOUR, A.A.. (2018). The effect of IAA on stress resistance of tobacco seedlings (Nicotiana plumbaginifolia) under in vitro culture conditions. JOURNAL OF CELL & TISSUE, 9(1 ), 86-101. SID. https://sid.ir/paper/395959/en

Vancouver: Copy

GHASEMI S., EHSANPOUR A.A.. The effect of IAA on stress resistance of tobacco seedlings (Nicotiana plumbaginifolia) under in vitro culture conditions. JOURNAL OF CELL & TISSUE[Internet]. 2018;9(1 ):86-101. Available from: https://sid.ir/paper/395959/en

IEEE: Copy

S. GHASEMI, and A.A. EHSANPOUR, “The effect of IAA on stress resistance of tobacco seedlings (Nicotiana plumbaginifolia) under in vitro culture conditions,” JOURNAL OF CELL & TISSUE, vol. 9, no. 1 , pp. 86–101, 2018, [Online]. Available: https://sid.ir/paper/395959/en

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