miR-33a, miR-429 and miR-200c with EMT pathway was investigated in non-small cell lung cancer

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Golshan Rad gh.; ZARE KARIZI SH.; KARIMIPOOR M.

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ISSN: 2588-4824

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Journal: JOURNAL OF CELL & TISSUE Year:2019 | Volume:10 | Issue:3 Page(s): 143-151

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Title

miR-33a, miR-429 and miR-200c with EMT pathway was investigated in non-small cell lung cancer

Author(s)

Golshan Rad gh. | ZARE KARIZI SH. | KARIMIPOOR M. | Issue Writer Certificate

Keywords

non-small cell lung cancerQ1

Epithelial–mesenchymal transition.miRNAQ1

Abstract

Aim: In this study, the correlation of miR-33a, miR-429 and miR-200c with EMT pathway was investigated in the non-small cell lung cancer. Material and methods: Real-Time PCR was used to evaluate the expression levels of the mentioned miRNA in 30 non-small cell lung tumor tissues (66% of cells were in stages I, II and the rest were in stage III). RNA extraction was performed and cDNA synthesized using stem-loop primers. The quantitative evolution of gene expression were performed using specific primers and data was analyzed by 2 Results: The evaluation of expression changes for miR-33a showed that 11 samples were upregulated. miR-429 and miR-200c showed an increased expressions in 12 and 18 tumor samples, respectively. There was no significant difference between the levels of expression (P≤ 0. 05). Conclusion: There was no significant change in the level of expression of miR-33a, miR-429 and miR-200c. These results could be due to sample size or the early stage of samples.

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APA: Copy

Golshan Rad, gh., ZARE KARIZI, SH., & KARIMIPOOR, M.. (2019). miR-33a, miR-429 and miR-200c with EMT pathway was investigated in non-small cell lung cancer. JOURNAL OF CELL & TISSUE, 10(3 ), 143-151. SID. https://sid.ir/paper/395963/en

Vancouver: Copy

Golshan Rad gh., ZARE KARIZI SH., KARIMIPOOR M.. miR-33a, miR-429 and miR-200c with EMT pathway was investigated in non-small cell lung cancer. JOURNAL OF CELL & TISSUE[Internet]. 2019;10(3 ):143-151. Available from: https://sid.ir/paper/395963/en

IEEE: Copy

gh. Golshan Rad, SH. ZARE KARIZI, and M. KARIMIPOOR, “miR-33a, miR-429 and miR-200c with EMT pathway was investigated in non-small cell lung cancer,” JOURNAL OF CELL & TISSUE, vol. 10, no. 3 , pp. 143–151, 2019, [Online]. Available: https://sid.ir/paper/395963/en

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