JOURNAL OF CELL & TISSUE
Year:2019 | Volume:10 | Issue:3|Papers Count:6

im: This study was designed to investigate the use of different levels of soybean lecithin in the Tris extender on semen quality and sex ratio using real-time qPCR technique. Material and Methods: Sampling of 4 Holstien bulls was performed once a week, then the appropriate samples were mixed. Samples of sperm (in 4 replications) were divided into three groups: zero, one and two percentage soybean lecithin. The qualitative parameters and the sex ratio of the semen were determined. Swim up technique was used to wash the semen and remove the dead cells. Moreover, the PLP and SRY genes were propagated to separate the specific fragments of X-and Y-chromosomes sequences. In this procedure, PAR was used as a housekeeping gene to normalize the gene expression data in real-time qPCR. Results: The results showed that swim up technique and different levels of soybean lecithin improve the semen quality. In addition, swim up technique significantly reduced PLP gene expression (0. 75± 0. 11), against increased SRY gene expression (1. 37± 0. 13). However, using of different levels of soybean lecithin did not change the sex ratio of the sperms. Conclusion: In general, soybean lecithin is an appropriate substitute to animal sources and improves the quality of semen. Due to the limitations of using animal resources in bull extenders, the use of soybean lecithin as a plant source is suggested.

Aim: In this study, the correlation of miR-33a, miR-429 and miR-200c with EMT pathway was investigated in the non-small cell lung cancer. Material and methods: Real-Time PCR was used to evaluate the expression levels of the mentioned miRNA in 30 non-small cell lung tumor tissues (66% of cells were in stages I, II and the rest were in stage III). RNA extraction was performed and cDNA synthesized using stem-loop primers. The quantitative evolution of gene expression were performed using specific primers and data was analyzed by 2 Results: The evaluation of expression changes for miR-33a showed that 11 samples were upregulated. miR-429 and miR-200c showed an increased expressions in 12 and 18 tumor samples, respectively. There was no significant difference between the levels of expression (P≤ 0. 05). Conclusion: There was no significant change in the level of expression of miR-33a, miR-429 and miR-200c. These results could be due to sample size or the early stage of samples.

Aim: This study aimed to produce recombinant hybrid protein, DT389GCSF, in E. coli BL-21(DE3) in a soluble and active form and to purify it and evaluate its cytotoxicity. Material and Methods: The protein, His6-tagged DT389GCSF was expressed in E. coli BL-21(DE3) in a soluble form at 28 ° C. Then it was purified by affinity chromatography on nickel sepharose column. Finally, the function of purified recombinant protein was evaluated by MTT assay on HL-60 cancer cell line. Results: The yield of expression and purification of DT389GCSF were determined at about 12 and 80 percent, respectively, using Image J software. The IC50 value upon 48 hours of exposure of DT389GCSF toward cell line was about 0. 00017± 0. 000019 M. Conclusion: By reducing the temperature and using the intracellular mechanism, the refolding of hybrid protein, DT389GCSF, can be observed in a proper and active form. As a result, in vitro production of relevant immunotoxins, by using this method, the steps of inclusion body production can be neglected.

Aim: The aim of this study was to investigate the effects of 12 weeks high-intensity interval training (HIT) and low-intensity continuous training (LIT) on RXRα gene expression in male wistar rats after a high fat-diet. Material and Methods: Subjects after 13 weeks high-fat diet were divided into three groups: control, HIT training and LIT training. Then experimental groups performed exercise training for 12 weeks. After training, RXRα gene expression was analyzed. Results: the results indicated RXRα gene expression in the HIT group was higher than the LIT group and also in the LIT group was higher than the control group. All of the observed differences were significant (P≤ 0/05). Conclusion: in total, 12 weeks HIT and LIT after 13 weeks high-fat diet may be effective in increment of RXRα gene expression that is an effective non-pharmacological strategy for atherogenic effects of obesity and overweight.

Aim: . The aim of the current study was to evaluate the effect of magnesium Nano Oxide on learning and memory and biochemical changes in the hippocampus of sleep-deprived rats. Material and Methods: In this study, male rats were used in control groups whereas others used rats were sleep deprived and the rats receiving Magnesium Nano Oxide with doses of 1, 5, and 10 mg/kg. Step-through apparatus was used to evaluate passive avoidance memory. Laboratory kit and glucometer were used for biochemical evaluation. Results: The results showed that the magnesium Nano oxide in 10 mg/kg/rat can result in increasing efficiency of memory in male rats that experienced memory impaired resulted from sleep deprivation. In addition to afore-mentioned behavioral changes, the investigator found that sleep deprivation decreases the level of Taurine Amino Acid. However, it can increase the blood sugar and cholinesterase enzyme activity. In addition, it didn't have significant effect on Brain-derived Neurotrophic factor. Upon application of Magnesium Nano oxide, the amount of blood sugar and collinstrase enzyme activity lowered, but the amount of Taurine Amino Acid remained unchanged. Conclusion: The findings show that in addition to behavioral effects, magnesium Nano oxide causes a change in the amount of biochemical factors affecting memory. That needs to be further investigated.

Aim: This study was aimed to preparation of sheep urinary bladder derived from biological scaffold by a combined (physical and chemical) method and evaluation of scaffold biocompatibility. Materials and Methods: Urinary bladder decellularization was performed by physical and chemical methods. In the physical method, the bladder fragments were incubated at-4 ° C for 24 h and intervaled the fragments each 6 h by placing for 10 min in 0. 1% sodium azide solution. After 24 h, the samples were placed at-20 and-40 ° C for 2 and 1 h, respectively. The bladder fragments were held in a cryotube and emerged in liquid nitrogen by five two-minute steps, and finally washed by the PBS buffer containing 0. 1% sodium azide. In the chemical decellularization, all of the physically treated bladder fragments were placed in a sodium dodecyl sulphate solution under slow stirring for 24 h. The samples were rinsed with sterile distilled water, sterilized with 75% ethanol and 0. 2% peracetic acid and finally were placed in PBS for 24 h. Results: The light and electron microscopey studies revealed the biocompatibility of seeded stem cells on the sheep bladder bioscaffold, in 3rd, 5th and 7th days. The most biocompatibility was observed in the end of 7th day. Conclusion: Decellularized urinary bladder scaffold revealed biocompatibility which could be considered as a potential nontoxic and biocompatible bioscaffold for application in tissue engineering regenerative medicine.