Optimization callus indication and Study of FLS and CHI gene expression in quercetin biosynthesis pathway in callus culture with UV-B radiation of Momordica charantia L.

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Fooladvand Z.; FAZELI NASAB B.

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Journal: Ecophytochemistry journal of Medicinal Plants Year:2019 | Volume:7 | Issue:2 (26) Page(s): 40-53

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Title

Optimization callus indication and Study of FLS and CHI gene expression in quercetin biosynthesis pathway in callus culture with UV-B radiation of Momordica charantia L.

Author(s)

Fooladvand Z. | FAZELI NASAB B. | Issue Writer Certificate

Keywords

BAPQ1

FlavonoidQ1

FLSQ1

QuercetinQ1

Momordica charantiaQ1

2Q1

4-D2Q1

Abstract

Quercetin is one of the most natural secondary metabolites, which found in fruits and leaves of Momordica charantia and characterized as an antioxidant, antiviral and anticancer compound. The present study aimed to optimize the callus induction and to determine the expression of flavonol synthase and chalcone isomerase genes in the pathway of Quercetin production under UV-B rays in bitter cucumber callus cultivation. The experiment was conducted in 2018 at Agricultural Biotechnology Institute of Zabol University. In this research different concentrations of plant hormones ((2, 4-D (0, 0. 25, 0. 5, 1 and 5 mg/L) and BAP (0. 2, 0. 5, 1 and 5 mg/L)) and explant (Leaf and Stem) was used to callus induction in Momordica charantia using complete random design with three replications. Effect of UV-B radiation in five groups: control, natural light, 5 min exposure (gene expression at 24 and 48 after treatment) and 10 min exposure (gene expression at 24 and 48 after treatment)) on the expression of Quercetin biosynthetic pathway genes including flavonol synthase (FLS) and chalcone isomerase (CHI) (by 2-Δ Δ CT method), phenyl ammonia-lyase (PAL) antioxidant activity (by cinnamic acid concentration), and Flavonoid content (by HPLC method)) in the selected best calluses. Analysis of variance were showed that different types and concentrations of plant hormones had difference significant on callus induction. Mean square of data showed that the most callus dry weight was obtained from 0. 5 mg/L 2, 4-D and 1 mg/L BAP for stem explant. The highest expression of FLS and CHI were observed within 24 hours after 10-min UV-B treatment, but it decreased at 48 h after treatment. The highest expression of PAL, Quercetin and Flavonoid were obtained within 48 hours after 5-min UV-B treatment. Comparison of irradiated and untreated calluses revealed that the average changes of relative expression of FLS and CHI were 1. 4 and 2. 4 times higher than control, respectively. Overall, the results were showed that UV-B radiation at the callus level had a positive effect on the expression of Flavonoids biosynthetic pathway genes.

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APA: Copy

Fooladvand, Z., & FAZELI NASAB, B.. (2019). Optimization callus indication and Study of FLS and CHI gene expression in quercetin biosynthesis pathway in callus culture with UV-B radiation of Momordica charantia L.. ECO-PHYTOCHEMICAL JOURNAL OF MEDICAL PLANTS, 7(2 (26) ), 40-53. SID. https://sid.ir/paper/396732/en

Vancouver: Copy

Fooladvand Z., FAZELI NASAB B.. Optimization callus indication and Study of FLS and CHI gene expression in quercetin biosynthesis pathway in callus culture with UV-B radiation of Momordica charantia L.. ECO-PHYTOCHEMICAL JOURNAL OF MEDICAL PLANTS[Internet]. 2019;7(2 (26) ):40-53. Available from: https://sid.ir/paper/396732/en

IEEE: Copy

Z. Fooladvand, and B. FAZELI NASAB, “Optimization callus indication and Study of FLS and CHI gene expression in quercetin biosynthesis pathway in callus culture with UV-B radiation of Momordica charantia L.,” ECO-PHYTOCHEMICAL JOURNAL OF MEDICAL PLANTS, vol. 7, no. 2 (26) , pp. 40–53, 2019, [Online]. Available: https://sid.ir/paper/396732/en

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