The effect of auxin type, explant orientation and light condition on induction of callus and the formation of hairy roots on leaf explants of medicinal plant Bitter melon (Momordica charantia L. )

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SHAFAEI M.; MOKHTARI A.; EBRAHIMI M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Journal: Journal of Plant Research Year:2020 | Volume:33 | Issue:1 Page(s): 00-00

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Title

The effect of auxin type, explant orientation and light condition on induction of callus and the formation of hairy roots on leaf explants of medicinal plant Bitter melon (Momordica charantia L. )

Author(s)

SHAFAEI M. | MOKHTARI A. | EBRAHIMI M. | Issue Writer Certificate

Keywords

Momordica charantiaQ1

CallusQ2

LightQ1

Explant OrientationQ1

Abstract

The bitter melon (Momordica charantia) is a medicinal herb from the Cucurbitaceae family. This herb is used as a traditional drug for the treatment of diabetes. This research was carried out with the aim of investigating the effect of the leaf orientation, auxin type and Light conditions on the percentage of Callus induction and the formation of hairy root from derived Callus. The leaf explants were cultured in two directions (up/ down side) in a medium containing 2 types of auxins at a concentration of 2 mg /l NAA and 2, 4-D, under dark and Light conditions. The highest callogenesis (95. 40%) and induction of roots (40. 60%) were obtained when the upper surface of the leaf placed on the Murashige and Skoog (MS) medium contained 2 mg /l 2, 4-D under the dark condition. These results indicate that dark condition has a significant effect on stimulating and increasing of Callus induction (P≤ 0. 05) and 2, 4-D was the better auxin compared to NAA for induction of Callus and subsequently roots on the leaf-derived Callus tissues.

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APA: Copy

SHAFAEI, M., MOKHTARI, A., & EBRAHIMI, M.. (2020). The effect of auxin type, explant orientation and light condition on induction of callus and the formation of hairy roots on leaf explants of medicinal plant Bitter melon (Momordica charantia L. ). JOURNAL OF PLANT RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 33(1 ), 00-00. SID. https://sid.ir/paper/394494/en

Vancouver: Copy

SHAFAEI M., MOKHTARI A., EBRAHIMI M.. The effect of auxin type, explant orientation and light condition on induction of callus and the formation of hairy roots on leaf explants of medicinal plant Bitter melon (Momordica charantia L. ). JOURNAL OF PLANT RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2020;33(1 ):00-00. Available from: https://sid.ir/paper/394494/en

IEEE: Copy

M. SHAFAEI, A. MOKHTARI, and M. EBRAHIMI, “The effect of auxin type, explant orientation and light condition on induction of callus and the formation of hairy roots on leaf explants of medicinal plant Bitter melon (Momordica charantia L. ),” JOURNAL OF PLANT RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 33, no. 1 , pp. 00–00, 2020, [Online]. Available: https://sid.ir/paper/394494/en

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