Effect of explants type, light and polysaccharides on micropropagation of Catasetum orchid (Catasetum fimbriatum, L. )

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MOHAMADI ELHAM; CHALAVI VIDA; MORADI HOSSEIN

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Journal: JOURNAL OF PLANT PROCESS AND FUNCTION Year:2019 | Volume:8 | Issue:30 Page(s): 171-184

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Title

Effect of explants type, light and polysaccharides on micropropagation of Catasetum orchid (Catasetum fimbriatum, L. )

Author(s)

MOHAMADI ELHAM | CHALAVI VIDA | MORADI HOSSEIN | Issue Writer Certificate

Keywords

Orchid catasetumQ1

RegenerationQ2

DarknessQ1

SucroseQ2

MannitolQ2

Explant typeQ1

Abstract

The aim of present study was to investigate the effect of explants types, in light and Darkness condition on propagation of lateral plantlets and also to investigate the effect of type and concentrations of carbohydrates on the Regeneration of Catasetum orchid plantlets in two experiments. First experiment for propagation of lateral plantlets was done with two types of explants, shoot tips and root tips, in combination with different lighting conditions (light and Darkness) in completely randomized design with 5 replications. According to obtained results, the production of leaves, roots, lateral plantlets and the intensity of explant necrosis were affected by explants types and environmental conditions of light and Darkness treatments and were statistically significant at (p ≤ 0. 01 & 0. 05). The highest mean number of leaves (21. 4), roots (5) were from shoot tip explants in light and the highest mean number of lateral plantlets (17. 8) was observed in root explants in Darkness. In second experiment, the effect of different concentrations of Sucrose and Mannitol was investigated on the Regeneration of Catasetum orchid plantlets. Experimental treatments included three levels of Sucrose, 20, 30, 40 g/l, two levels of Mannitol, 10. 08 and 22 g/l in combination with 20 g/l of Sucrose and Mannitol alone at 10. 08 g/l concentration. The highest mean number of formed lateral plantlets from each explant was 10. 48 which was obtained at 30 g/l Sucrose concentration. For production and growth of protocorm like body, all Mannitol concentrations were better than Sucrose and the highest protocorm like body mean number (1. 54) was produced in 10. 08 g/l Mannitol concentration. In general conclusion, the highest lateral plantlets Regeneration occurred from root explants in Darkness treatment by using Sucrose.

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APA: Copy

MOHAMADI, ELHAM, CHALAVI, VIDA, & MORADI, HOSSEIN. (2019). Effect of explants type, light and polysaccharides on micropropagation of Catasetum orchid (Catasetum fimbriatum, L. ). JOURNAL OF PLANT PROCESS AND FUNCTION, 8(30 ), 171-184. SID. https://sid.ir/paper/397079/en

Vancouver: Copy

MOHAMADI ELHAM, CHALAVI VIDA, MORADI HOSSEIN. Effect of explants type, light and polysaccharides on micropropagation of Catasetum orchid (Catasetum fimbriatum, L. ). JOURNAL OF PLANT PROCESS AND FUNCTION[Internet]. 2019;8(30 ):171-184. Available from: https://sid.ir/paper/397079/en

IEEE: Copy

ELHAM MOHAMADI, VIDA CHALAVI, and HOSSEIN MORADI, “Effect of explants type, light and polysaccharides on micropropagation of Catasetum orchid (Catasetum fimbriatum, L. ),” JOURNAL OF PLANT PROCESS AND FUNCTION, vol. 8, no. 30 , pp. 171–184, 2019, [Online]. Available: https://sid.ir/paper/397079/en

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