Extraction, purification and structural elucidation of the main water-soluble polysaccharide isolated from Acanthophyllum glandulosum roots

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Molaveisi M.; JAHANBIN K.

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Journal: JOURNAL OF FOOD RESEARCH (UNIVERSITY OF TABRIZ) Year:2018 | Volume:28 | Issue:2 Page(s): 85-100

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Title

Extraction, purification and structural elucidation of the main water-soluble polysaccharide isolated from Acanthophyllum glandulosum roots

Author(s)

Molaveisi M. | JAHANBIN K. | Issue Writer Certificate

Keywords

ExtractionQ2

PolysaccharideQ2

Acanthophyllum glandulosumQ1

PurificationQ3

Structural CharacterizationQ1

Abstract

A new water-soluble Polysaccharide with molecular weight of 35. 2 KDa and a specific optical rotation of +178. 5° was extract by hot water (80° C) from the roots of Acanthophyllum glandulosum. The Polysaccharide purified through DEAE-cellulose and Sephadex G-50 columns and the main fraction was collected and freeze-dried. Total yields of purified Polysaccharide were 3. 4% and %2. 3 respectively. The purified Polysaccharide had no absorption at 280 nm (Detected by UV spectrum), indicating the absence of protein and nucleic acid. Total carbohydrate content of purified Polysaccharide was 98. 2%. Monosaccharide analysis by GC revealed that Polysaccharide was composed of Glucose, Galactose, Arabinose and Mannose with a relative molar ratio of 2. 9: 6. 1: 0. 9: 1. 0 respectively. Its structural features were elucidated by a combination of chemical and analytical methods such as Methylation, Periodate Oxidation and Smith degradation, Partial acid hydrolysis, FT-IR and 13C and 1H NMR Spectroscopy. The data obtained indicate that purified Polysaccharide possessed a backbone of α-(1→ 6)-linked Galactose with branched attached to O-2 by α-1→ linked Mannose and at O-3 by α-(1→ 3)-linked Glucose and α-(1→ 3)-linked Arabinose.

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APA: Copy

Molaveisi, M., & JAHANBIN, K.. (2018). Extraction, purification and structural elucidation of the main water-soluble polysaccharide isolated from Acanthophyllum glandulosum roots. JOURNAL OF FOOD RESEARCH (UNIVERSITY OF TABRIZ), 28(2 ), 85-100. SID. https://sid.ir/paper/397149/en

Vancouver: Copy

Molaveisi M., JAHANBIN K.. Extraction, purification and structural elucidation of the main water-soluble polysaccharide isolated from Acanthophyllum glandulosum roots. JOURNAL OF FOOD RESEARCH (UNIVERSITY OF TABRIZ)[Internet]. 2018;28(2 ):85-100. Available from: https://sid.ir/paper/397149/en

IEEE: Copy

M. Molaveisi, and K. JAHANBIN, “Extraction, purification and structural elucidation of the main water-soluble polysaccharide isolated from Acanthophyllum glandulosum roots,” JOURNAL OF FOOD RESEARCH (UNIVERSITY OF TABRIZ), vol. 28, no. 2 , pp. 85–100, 2018, [Online]. Available: https://sid.ir/paper/397149/en

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