Optimization of the effective factors in E. coli growth producing recombinant β-NGF using response surface methodology

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Gholami tilko Pouria; Haji hassan Zahra; Nazari Navid; MOGHIMI HAMID

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF BIOTECHNOLOGY Year:2017 | Volume:8 | Issue:3 Page(s): 0-0

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

564

Download:

Cites:

Information Journal Paper

Title

Optimization of the effective factors in E. coli growth producing recombinant β-NGF using response surface methodology

Author(s)

Gholami tilko Pouria | Haji hassan Zahra | Nazari Navid | MOGHIMI HAMID | Issue Writer Certificate

Keywords

glycerolQ1

yeast extractQ1

MgCl2Q1

response surface methodology (RSM)Q1

overnight cultureQ1

Abstract

Production of recombinant proteins in Escherichia coli has been very common in recent decades. Many studies and experiments have been done in order to optimize the production and expression of recombinant proteins in E. coli. One strategy is using high cell density to increase recombinant protein production such as β-NGF in the cell. Therefore, in this study for the first time bacterial cell culture in high cell density was done using glycerol and yeast extract as carbon and nitrogen sources and MgCl2 as a growth effective factor. Also the effects of overnight culture conditions on bacterial growth were evaluated. Meanwhile culture conditions were optimized using response surface methodology (RSM) and the optimum conditions were as follows: 18/23 g/lit glycerol, 14. 44 g/lit yeast extract and 10mM MgCl2. Also the obtained results indicated that the 14 hours incubation at 37 ° C and 180 rpm were optimum conditions for the overnight culture. Our results showed that the rate of cell growth and recombinant β-NGF production in optimized condition is significantly higher than in basic medium.

Cites

No record.

References

No record.

Cite

APA: Copy

Gholami tilko, Pouria, Haji hassan, Zahra, Nazari, Navid, & MOGHIMI, HAMID. (2017). Optimization of the effective factors in E. coli growth producing recombinant β-NGF using response surface methodology. JOURNAL OF BIOTECHNOLOGY, 8(3 ), 0-0. SID. https://sid.ir/paper/406216/en

Vancouver: Copy

Gholami tilko Pouria, Haji hassan Zahra, Nazari Navid, MOGHIMI HAMID. Optimization of the effective factors in E. coli growth producing recombinant β-NGF using response surface methodology. JOURNAL OF BIOTECHNOLOGY[Internet]. 2017;8(3 ):0-0. Available from: https://sid.ir/paper/406216/en

IEEE: Copy

Pouria Gholami tilko, Zahra Haji hassan, Navid Nazari, and HAMID MOGHIMI, “Optimization of the effective factors in E. coli growth producing recombinant β-NGF using response surface methodology,” JOURNAL OF BIOTECHNOLOGY, vol. 8, no. 3 , pp. 0–0, 2017, [Online]. Available: https://sid.ir/paper/406216/en

Related Journal Papers