Investigating the optimal conditions of primary cell culture of kidney tissue from Caspian white fish (Rutilus kutum) using explant method

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Nikghorban Samaneh; HAGHIGHI KARSIDANI SOMAYEH; Ghasemi Mohaddes

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Journal: JOURNAL OF MARINE BIOLOGY Year:2019 | Volume:11 | Issue:41 Page(s): 79-90

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Title

Investigating the optimal conditions of primary cell culture of kidney tissue from Caspian white fish (Rutilus kutum) using explant method

Author(s)

Nikghorban Samaneh | HAGHIGHI KARSIDANI SOMAYEH | Ghasemi Mohaddes | Issue Writer Certificate

Keywords

Primary cell cultureQ1

Kidney tissueQ1

Rutilus kutumQ2

Abstract

Cell culture involves a process where cells from different types of tissues are replicated in vitro Tissue culture and development of the cell lines from fish is used to isolate viruses, toxicology, Oncology, cellular physiology and gene expression. In this study, Primary cell culture of kidney were prepared using a total of 10 Rutilus kutum with average weight of 12 ± 1 g and the mean length of 11 ± 1 cm were by explanting method. Tissue Pieces were explanted in cell culture flasks containing L-15 medium with antibiotics (penicillin, styrpthomycin) under different temperature conditions (21, 24 and 28 ° C) and fetal bovine serum levels (5, 10 and 20%) then cultured in two ranges ofpH (6. 8 and 7. 2). The results showed that the preparation of Primary cell culture of Kidney tissue of Caspian white fish (Rutilus kutum) at 24 ° C and 20% of the fetal bovine serum was better than other treatments and the cell migration rate was calculated as 88. 37%. So far, 8 passages from these cells have been carried out, which could lead to the production of a cell line from the kidney of Rutilus kutum in the near future.

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APA: Copy

Nikghorban, Samaneh, HAGHIGHI KARSIDANI, SOMAYEH, & Ghasemi, Mohaddes. (2019). Investigating the optimal conditions of primary cell culture of kidney tissue from Caspian white fish (Rutilus kutum) using explant method. JOURNAL OF MARINE BIOLOGY, 11(41 ), 79-90. SID. https://sid.ir/paper/406766/en

Vancouver: Copy

Nikghorban Samaneh, HAGHIGHI KARSIDANI SOMAYEH, Ghasemi Mohaddes. Investigating the optimal conditions of primary cell culture of kidney tissue from Caspian white fish (Rutilus kutum) using explant method. JOURNAL OF MARINE BIOLOGY[Internet]. 2019;11(41 ):79-90. Available from: https://sid.ir/paper/406766/en

IEEE: Copy

Samaneh Nikghorban, SOMAYEH HAGHIGHI KARSIDANI, and Mohaddes Ghasemi, “Investigating the optimal conditions of primary cell culture of kidney tissue from Caspian white fish (Rutilus kutum) using explant method,” JOURNAL OF MARINE BIOLOGY, vol. 11, no. 41 , pp. 79–90, 2019, [Online]. Available: https://sid.ir/paper/406766/en

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