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Information Journal Paper

Title

Investigation of Gene Expression Related to Stress in Native and Cross Breed Sheep

Pages

  57-72

Abstract

 Inroduction & Objective: Increasing in temperature and drying of pastures cause water and nutritional Stress to animals. In this study, resistance control genes in crossbred and Native Sheep were examined. Material and Methods: In this study, ten cross of Romanov native male lambs and ten purebred male native lambs in 4 treatments and 5 replications in each group were used. They are divided into two groups with normal and water restriction condition. At the end of the experiment, liver tissue samples were collected from slaughtered animals and it was kept in liquid nitrogen, to investigation of Gene Expression whole RNA was extracted and purified and the cDNA was synthesis. In the end Real Time PCR reaction were performed using specific primers. For statistical analysis we used randomized design as factorial analysis method using SAS 9. 2. Results: By extracting the genes, it was found that the expression of GPX1, TFAM, SIRT3, PRKAA1, NRT2 genes increased under Stress in Native Sheep compared to Crossbred Sheep. Conclusion: The expression of GPX1, TFAM, NRF2, SIRT, PKAA1 genes in the liver of two groups of native and crossbred in normal and Stress conditions indicate that Under Stress, these four groups of genes act as oxidative process and store energy and prevent energy loss and protect the cell. Due to the genetic compatibility of Native Sheep with the drought conditions of the region, it makes the animal more resistant to heat and disease.

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    APA: Copy

    Rouhbakhsh, N., HEDAYAT EVRIGH, N., SEYED SHARIFI, R., & SHAKOURI, M.D.. (2021). Investigation of Gene Expression Related to Stress in Native and Cross Breed Sheep. JOURNAL OF ANIMAL PHYSIOLOGY AND DEVELOPMENT (QUARTERLY JOURNAL OF BIOLOGICAL SCIENCES), 14(3 (54) ), 57-72. SID. https://sid.ir/paper/410976/en

    Vancouver: Copy

    Rouhbakhsh N., HEDAYAT EVRIGH N., SEYED SHARIFI R., SHAKOURI M.D.. Investigation of Gene Expression Related to Stress in Native and Cross Breed Sheep. JOURNAL OF ANIMAL PHYSIOLOGY AND DEVELOPMENT (QUARTERLY JOURNAL OF BIOLOGICAL SCIENCES)[Internet]. 2021;14(3 (54) ):57-72. Available from: https://sid.ir/paper/410976/en

    IEEE: Copy

    N. Rouhbakhsh, N. HEDAYAT EVRIGH, R. SEYED SHARIFI, and M.D. SHAKOURI, “Investigation of Gene Expression Related to Stress in Native and Cross Breed Sheep,” JOURNAL OF ANIMAL PHYSIOLOGY AND DEVELOPMENT (QUARTERLY JOURNAL OF BIOLOGICAL SCIENCES), vol. 14, no. 3 (54) , pp. 57–72, 2021, [Online]. Available: https://sid.ir/paper/410976/en

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