The Effect of Adding Different Level of Fennel Extracted on the Fertility of Drosophila melanogaster and its Effect on the Density of Probiotics in its Intestine Using Qreal-Time PCR Technique

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REZAEI A.H.; GHANE M.; Akhshabi Sh.

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Journal: JOURNAL OF ANIMAL PHYSIOLOGY AND DEVELOPMENT (QUARTERLY JOURNAL OF BIOLOGICAL SCIENCES) Year:2021 | Volume:14 | Issue:2 (53) Page(s): 1-23

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Title

The Effect of Adding Different Level of Fennel Extracted on the Fertility of Drosophila melanogaster and its Effect on the Density of Probiotics in its Intestine Using Qreal-Time PCR Technique

Author(s)

REZAEI A.H. | GHANE M. | Akhshabi Sh. | Issue Writer Certificate

Keywords

Drosophila melanogasterQ1

ProbioticsQ1

Fennel extractionQ1

ExpressionQ1

Abstract

Inroduction & Objective: The aim of this study, was additive extraxted fennel in the medium for increasing reproductive performance, concentration of probiotices by using qRT-PCR technique. Materials and Methods: In the present study, fennel extracted was divided into different concentrations. Concentrations including 25, 50 and 75 µ g /ml were added to the culture medium. In addition, a control sample was used to confirm the results. Parameters of mating latency and copulation duration, was measured by coronometr instrument. Fecundity and fertility also were count by Loop on the medium. The flies were studied for two months. Results: In this study, concentration of 25, 50, 75 µ g/ ml and control group was used. The results showed concentration of 75µ g/ml was significant decreasing mating latency and increasing copulation duration, fecundity and fertility parameters were showed. Moreover were confirmed lactobacilus brevis by PCR technique In addition using qRT-PCR technique and confirmed higher Expression in 16srDNA when used 75µ g/ml concentration. Conclusion: According to the present study, the higher fennel extracted has been effective on gene Expression in Probiotics and also on the reproductive performance of flies showed.

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APA: Copy

REZAEI, A.H., GHANE, M., & Akhshabi, Sh.. (2021). The Effect of Adding Different Level of Fennel Extracted on the Fertility of Drosophila melanogaster and its Effect on the Density of Probiotics in its Intestine Using Qreal-Time PCR Technique. JOURNAL OF ANIMAL PHYSIOLOGY AND DEVELOPMENT (QUARTERLY JOURNAL OF BIOLOGICAL SCIENCES), 14(2 (53) ), 1-23. SID. https://sid.ir/paper/411668/en

Vancouver: Copy

REZAEI A.H., GHANE M., Akhshabi Sh.. The Effect of Adding Different Level of Fennel Extracted on the Fertility of Drosophila melanogaster and its Effect on the Density of Probiotics in its Intestine Using Qreal-Time PCR Technique. JOURNAL OF ANIMAL PHYSIOLOGY AND DEVELOPMENT (QUARTERLY JOURNAL OF BIOLOGICAL SCIENCES)[Internet]. 2021;14(2 (53) ):1-23. Available from: https://sid.ir/paper/411668/en

IEEE: Copy

A.H. REZAEI, M. GHANE, and Sh. Akhshabi, “The Effect of Adding Different Level of Fennel Extracted on the Fertility of Drosophila melanogaster and its Effect on the Density of Probiotics in its Intestine Using Qreal-Time PCR Technique,” JOURNAL OF ANIMAL PHYSIOLOGY AND DEVELOPMENT (QUARTERLY JOURNAL OF BIOLOGICAL SCIENCES), vol. 14, no. 2 (53) , pp. 1–23, 2021, [Online]. Available: https://sid.ir/paper/411668/en

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