Cloning, expression and purification of vibrioblock proteins as candidates for cholera

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Yaghobi Moghaddam Mohammadali; Saeedinia Alireza; Samimi Nemati Afshin; Dehghan Esmatabadi Mohammad Javad

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Journal Paper

Paper Information

Journal: NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL Year:2021 | Volume:11 | Issue:43 Page(s): 93-102

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Title

Cloning, expression and purification of vibrioblock proteins as candidates for cholera

Author(s)

Yaghobi Moghaddam Mohammadali | Saeedinia Alireza | Samimi Nemati Afshin | Dehghan Esmatabadi Mohammad Javad | Issue Writer Certificate

Keywords

choleraQ1

candidate vaccineQ1

Recombinant proteinQ2

CloningQ2

recombinant DNAQ1

Iau ScienceQ1

Abstract

Aim and Background: With the advent of cloning technology and recombinant DNA, it became possible to produce drugs and vaccines that were not possible in the past. Recombinant protein occupies a large part of the biopharmaceutical industry, one of the most important of which is vaccination. Material and Methods: In this study, with the aim of obtaining a protein candidate for cholera vaccine, first cloning of a plasmid designed for the bacterial host E. coli DE3 Rossetagami was transformed, then expression was induced using IPTG and after protein expression was examined by SDS-PAGE gel electrophoresis, and then the protein was purified by nickel-sepharose (NI-NTA) chromatography column. Result: The results show that the purified protein has a molecular weight of 31 kDa and the amount of purified protein is 1 mg. Conclusion: Based on previous studies as well as the results of this study, the resulting protein can be considered as a suitable candidate for the cholera vaccine.

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APA: Copy

Yaghobi Moghaddam, Mohammadali, Saeedinia, Alireza, Samimi Nemati, Afshin, & Dehghan Esmatabadi, Mohammad Javad. (2021). Cloning, expression and purification of vibrioblock proteins as candidates for cholera. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, 11(43 ), 93-102. SID. https://sid.ir/paper/414876/en

Vancouver: Copy

Yaghobi Moghaddam Mohammadali, Saeedinia Alireza, Samimi Nemati Afshin, Dehghan Esmatabadi Mohammad Javad. Cloning, expression and purification of vibrioblock proteins as candidates for cholera. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL[Internet]. 2021;11(43 ):93-102. Available from: https://sid.ir/paper/414876/en

IEEE: Copy

Mohammadali Yaghobi Moghaddam, Alireza Saeedinia, Afshin Samimi Nemati, and Mohammad Javad Dehghan Esmatabadi, “Cloning, expression and purification of vibrioblock proteins as candidates for cholera,” NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, vol. 11, no. 43 , pp. 93–102, 2021, [Online]. Available: https://sid.ir/paper/414876/en

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