DHPLC APPLICATIONS:FINDING DNA VARIATION ON THE Y CHROMOSOME

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AYUB Q.; MOHYUDDIN A.; KHALIQ SH.; HAMEED A.; MEHDI Q.; TYLER-
SMITH CH.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Biotechnology Year:2003 | Volume:1 | Issue:1 Page(s): 26-30

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Title

DHPLC APPLICATIONS:FINDING DNA VARIATION ON THE Y CHROMOSOME

Author(s)

Keywords

DHPLC

Y-POLYMORPHISMS

Y SNPS

POPULATION GENETICS

PAKISTAN.

Abstract

Denaturing High-Performance Liquid Chromatography (DHPLC) is a recently developed technique for the detection of single nucleotide polymorphisms (SNPs) and mutations. It involves the comparison between two or more DNAs as a mixture of denatured and reannealed PCR products. The methodology is based on the principle of reversed phase liquid chromatography and uses a unique DNA separation matrix. The exquisite sensitivity of the technique is determined by adjusting the oven temperature. Elution of DNA fragments is dependant on the chain length and sequence and can be predicted by computation. Under partially denaturing conditions heteroduplices formed upon mixing, denaturing, and reannealing of two, or more, chromosomes that differ in sequence, are retained less than their corresponding homodupulices and sequence variation is recognized by the appearance of two, or more, peaks in the chromatographs. Numerous SNPs have been identified on the non-recombinant portion of the Y chromosome by using this technique. To investigate the DNA variation within PAKISTAN.15 Y-SNPs, an Alu insertion, a LINE1 insertion and the 12f2 deletion, mapping on the non-recombining portion of the human Y chromosome, were typed in 834 PAKISTAN. males. The combination of these biallelic markers identified 11 stable Y chromosomal lineages or Y ‘haplogroups’ in the PAKISTAN. population. Haplogroup frequencies were generally similar tothose in neighboring geographical areas and indicate that there was a common pool of Y lineages within Pakistan that are predominantly from West and Central Asia.

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APA: Copy

AYUB, Q., MOHYUDDIN, A., KHALIQ, SH., HAMEED, A., MEHDI, Q., & TYLER- SMITH, CH.. (2003). DHPLC APPLICATIONS:FINDING DNA VARIATION ON THE Y CHROMOSOME. IRANIAN JOURNAL OF BIOTECHNOLOGY, 1(1), 26-30. SID. https://sid.ir/paper/528241/en

Vancouver: Copy

AYUB Q., MOHYUDDIN A., KHALIQ SH., HAMEED A., MEHDI Q., TYLER- SMITH CH.. DHPLC APPLICATIONS:FINDING DNA VARIATION ON THE Y CHROMOSOME. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2003;1(1):26-30. Available from: https://sid.ir/paper/528241/en

IEEE: Copy

Q. AYUB, A. MOHYUDDIN, SH. KHALIQ, A. HAMEED, Q. MEHDI, and CH. TYLER- SMITH, “DHPLC APPLICATIONS:FINDING DNA VARIATION ON THE Y CHROMOSOME,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 1, no. 1, pp. 26–30, 2003, [Online]. Available: https://sid.ir/paper/528241/en

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