GROWTH AND ISOLATION OF HUMAN CYTOMEGALOVIRUS ON A NEW HUMAN FETAL FORESKIN FIBROBLAST-DERIVED CELL LINE IN IRAN

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AMINI BAVIL OULIAEI S.; SABAHI F.; ROUSTAEI M.H.; KARIMI ARZENANI M.; SARAMI FOROUSHANI R.; ADELI A.; SAMADI BAHRAMI Z.

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Journal Paper

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Journal: Iranian Journal of Biotechnology Year:2003 | Volume:1 | Issue:4 Page(s): 247-251

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Title

GROWTH AND ISOLATION OF HUMAN CYTOMEGALOVIRUS ON A NEW HUMAN FETAL FORESKIN FIBROBLAST-DERIVED CELL LINE IN IRAN

Author(s)

Keywords

HUMAN FETAL FORESKIN FIBROBLAST (HFFF);

Abstract

Cell culture technique has been used for detection and confirmation of many different viruses in clinical samples. Although, new diagnostic methods have been developed for viral infections, traditional cell culture technique is still regarded as the “gold standard” for several infectious agents such as human cytomegalovirus (HCMV). In the present study, a new human fetal foreskin fibroblast (HFFF)-derived cell line was obtained from a normal Iranian male fetus at the end of first trimester at National Cell Bank of Iran (NCBI) and its ability for HCMV growth was investigated. Thirteen treated urine samples from renal transplant recipients were inoculated onto HFFF monolayer. HCMV growth was monitored and confirmed by typical CMV cytopathic effect (CPE), histological staining, immunological staining and polymerase chain reaction (PCR)-based restriction fragment length polymorphism (RFLP) method and sequencing. All of the abovementioned detection methods were confirmed that new HFFF-derived cell line is a sensitive line for growth of HCMV. This cell line has been named HFFF-PI6 and is available with accession number NCBI C170 at NCBI of Pasteur Institute of Iran.

Cites

PRE-SYMPTOMATIC HUMAN CYTOMEGALOVIRUS DISEASE DIAGNOSIS IN RENAL TRANSPLANT RECIPIENTS BY THE VIRUS DNA PCR

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APA: Copy

AMINI BAVIL OULIAEI, S., SABAHI, F., ROUSTAEI, M.H., KARIMI ARZENANI, M., SARAMI FOROUSHANI, R., ADELI, A., & SAMADI BAHRAMI, Z.. (2003). GROWTH AND ISOLATION OF HUMAN CYTOMEGALOVIRUS ON A NEW HUMAN FETAL FORESKIN FIBROBLAST-DERIVED CELL LINE IN IRAN. IRANIAN JOURNAL OF BIOTECHNOLOGY, 1(4), 247-251. SID. https://sid.ir/paper/528246/en

Vancouver: Copy

AMINI BAVIL OULIAEI S., SABAHI F., ROUSTAEI M.H., KARIMI ARZENANI M., SARAMI FOROUSHANI R., ADELI A., SAMADI BAHRAMI Z.. GROWTH AND ISOLATION OF HUMAN CYTOMEGALOVIRUS ON A NEW HUMAN FETAL FORESKIN FIBROBLAST-DERIVED CELL LINE IN IRAN. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2003;1(4):247-251. Available from: https://sid.ir/paper/528246/en

IEEE: Copy

S. AMINI BAVIL OULIAEI, F. SABAHI, M.H. ROUSTAEI, M. KARIMI ARZENANI, R. SARAMI FOROUSHANI, A. ADELI, and Z. SAMADI BAHRAMI, “GROWTH AND ISOLATION OF HUMAN CYTOMEGALOVIRUS ON A NEW HUMAN FETAL FORESKIN FIBROBLAST-DERIVED CELL LINE IN IRAN,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 1, no. 4, pp. 247–251, 2003, [Online]. Available: https://sid.ir/paper/528246/en

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