CLONING AND SEQUENCING OF DESULFURIZATION OPERON FROM A NEWLY ISOLATED BACTERIUM RHODOCOCCUS FMF

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AKBARZADEH S.; RASTGAR JAZI F.; RAHEB J.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF BIOTECHNOLOGY Year:2004 | Volume:2 | Issue:1 Page(s): 19-24

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Title

CLONING AND SEQUENCING OF DESULFURIZATION OPERON FROM A NEWLY ISOLATED BACTERIUM RHODOCOCCUS FMF

Author(s)

AKBARZADEH S. | RASTGAR JAZI F. | RAHEB J. | Issue Writer Certificate

Keywords

RHODOCOCCUS SPP.

BIODESULFURIZATION

DIBENZOTHIOPHEN.

Abstract

A native strain of Rhodococcus FMF was isolated from
soil samples collected from Tabriz petroleum refinery
area in Iran. The presence of sox operon in the
genomic DNA and the ability of bacteria to consume
dibenzothiophen (DBT) as sulfur source were
assessed. DNA was amplified by PCR and
subsequently cloned into pTZ57R cloning vector.
Diffrent restriction endonucleases; EcoRI, HindIII,
EcoRI/HindIII and XhoI were used to prove the accuracy
of cloning. Acquired clone was named
pTZAB57R. Subsequently, the relevant A and B genes
involved in DBT consumption were sequenced and
compared with the map of Rhodococcus erythropolis
IGTS8 desulfurization pathway genes. Results show
that the desulfurization operon in the native isolated
Rhodococcus FMF bacterium is completely conserved.

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APA: Copy

AKBARZADEH, S., RASTGAR JAZI, F., & RAHEB, J.. (2004). CLONING AND SEQUENCING OF DESULFURIZATION OPERON FROM A NEWLY ISOLATED BACTERIUM RHODOCOCCUS FMF. IRANIAN JOURNAL OF BIOTECHNOLOGY, 2(1), 19-24. SID. https://sid.ir/paper/528248/en

Vancouver: Copy

AKBARZADEH S., RASTGAR JAZI F., RAHEB J.. CLONING AND SEQUENCING OF DESULFURIZATION OPERON FROM A NEWLY ISOLATED BACTERIUM RHODOCOCCUS FMF. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2004;2(1):19-24. Available from: https://sid.ir/paper/528248/en

IEEE: Copy

S. AKBARZADEH, F. RASTGAR JAZI, and J. RAHEB, “CLONING AND SEQUENCING OF DESULFURIZATION OPERON FROM A NEWLY ISOLATED BACTERIUM RHODOCOCCUS FMF,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 2, no. 1, pp. 19–24, 2004, [Online]. Available: https://sid.ir/paper/528248/en

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