CASPASE DEPENDENT APOPTOSIS INDUCED BY CLADRIBINE IN THE ESTROGEN RECEPTOR NEGATIVE BREAST CANCER CELL LINE, MDA-MB468

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HASHEMI M.; KARAMI TEHRANI FATEMEH; FARZAMI B.

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Journal Paper

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Journal: Journal of Sciences, Islamic Republic of Iran Year:2003 | Volume:14 | Issue:4 Page(s): 303-310

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Title

CASPASE DEPENDENT APOPTOSIS INDUCED BY CLADRIBINE IN THE ESTROGEN RECEPTOR NEGATIVE BREAST CANCER CELL LINE, MDA-MB468

Author(s)

HASHEMI M. | KARAMI TEHRANI FATEMEH | FARZAMI B. | Issue Writer Certificate

Keywords

CLADRIBINE; APOPTOSIS; MDA-MB468; CASPASE-3; CASPASE-9

Abstract

The purpose of the present study is to investigate the cytotoxicity/apoptotic effect of 2-chloro-2′-deoxyadenosine, cladribine, (2-CdA) in the human breast cancer cell line, MDA-MB468 (estrogen receptor negative, ER−). MTT [3-(4,5- dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide] assay, annexin VFluorescein/ PI and Hoechst 33258 staining were used to detect cytotoxicity and cell apoptosis. The activation of caspase-3 and -9 was assayed using caspase activation assay kits. Gel electrophoresis was performed to detect DNA fragmentation. Treatment of MDA-MB468 cells with different concentrations of 2-CdA (50, 100 and 500 μM) resulted in a significant increase in the cell death. Annexin V-Fluorescein/PI and Hoechst 33258 staining revealed that the cell death was mainly of apoptotic type. DNA laddering profile was also obtained in the treated MDA-MB468 cells using DNA fragmentation analysis. A significant (p<0.05) increase in the activity of caspase-3 and -9 was observed. Pre-treatment of the cells with kinase inhibitor, 5′-amino-5′-deoxyadenosine inhibited the cytotoxicity effect of 2-CdA. This suggests that intracellular phosphorylation activation reaction plays a key role in the 2-CdA-induced apoptosis. In conclusion, this study showed that high dose of cladribine has an apoptotic effect on ER−MDA-MB468 breast cancer cells and its intracellular phosphorylation is necessary for cytotoxicity.

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APA: Copy

HASHEMI, M., KARAMI TEHRANI, FATEMEH, & FARZAMI, B.. (2003). CASPASE DEPENDENT APOPTOSIS INDUCED BY CLADRIBINE IN THE ESTROGEN RECEPTOR NEGATIVE BREAST CANCER CELL LINE, MDA-MB468. JOURNAL OF SCIENCES ISLAMIC REPUBLIC OF IRAN, 14(4), 303-310. SID. https://sid.ir/paper/529078/en

Vancouver: Copy

HASHEMI M., KARAMI TEHRANI FATEMEH, FARZAMI B.. CASPASE DEPENDENT APOPTOSIS INDUCED BY CLADRIBINE IN THE ESTROGEN RECEPTOR NEGATIVE BREAST CANCER CELL LINE, MDA-MB468. JOURNAL OF SCIENCES ISLAMIC REPUBLIC OF IRAN[Internet]. 2003;14(4):303-310. Available from: https://sid.ir/paper/529078/en

IEEE: Copy

M. HASHEMI, FATEMEH KARAMI TEHRANI, and B. FARZAMI, “CASPASE DEPENDENT APOPTOSIS INDUCED BY CLADRIBINE IN THE ESTROGEN RECEPTOR NEGATIVE BREAST CANCER CELL LINE, MDA-MB468,” JOURNAL OF SCIENCES ISLAMIC REPUBLIC OF IRAN, vol. 14, no. 4, pp. 303–310, 2003, [Online]. Available: https://sid.ir/paper/529078/en

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