PURIFICATION AND SOME PARTIAL CHARACTERIZATION OF PEROXIDASE ISOENZYME FROM BRASSICA OLERACEA CAPITATA L.

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TABATABAEI YAZDI S.M.; KHALEGHPARAST S.; MONSEF H.R.

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Journal Paper

Paper Information

Journal: Journal of Sciences, Islamic Republic of Iran Year:2002 | Volume:13 | Issue:2 Page(s): 107-112

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Title

PURIFICATION AND SOME PARTIAL CHARACTERIZATION OF PEROXIDASE ISOENZYME FROM BRASSICA OLERACEA CAPITATA L.

Author(s)

TABATABAEI YAZDI S.M. | KHALEGHPARAST S. | MONSEF H.R. | Issue Writer Certificate

Keywords

BRASSICA; CABBAGE; PURIFICATION; PEROXIDASE

Abstract

Acetone fractionated peroxidase from crude extract of Brassica oleracea leaves (Cabbage) was purified in three steps on chromatographic columns, using Sp-Sepharose, DEAE-Sepharose and Con A-Sepharose. The specific activity of purified main isoenzyme (BOC-POD) is 1887 u/mg protein with RZ: 3.1, which is 172 times more than the RZ of crude extract with 4.3% recovery. The molecular weight of BOC-POD is about 45,000 Dalton. Maximum pH, thermal activity and stability of this purified enzyme are also determined. Km of this isoenzyme was measured by Linewearver-Burk curve for O-dianisidine towards H2O2. This purified enzyme could be used in manufacturing diagnostic kits.

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APA: Copy

TABATABAEI YAZDI, S.M., KHALEGHPARAST, S., & MONSEF, H.R.. (2002). PURIFICATION AND SOME PARTIAL CHARACTERIZATION OF PEROXIDASE ISOENZYME FROM BRASSICA OLERACEA CAPITATA L.. JOURNAL OF SCIENCES ISLAMIC REPUBLIC OF IRAN, 13(2), 107-112. SID. https://sid.ir/paper/529081/en

Vancouver: Copy

TABATABAEI YAZDI S.M., KHALEGHPARAST S., MONSEF H.R.. PURIFICATION AND SOME PARTIAL CHARACTERIZATION OF PEROXIDASE ISOENZYME FROM BRASSICA OLERACEA CAPITATA L.. JOURNAL OF SCIENCES ISLAMIC REPUBLIC OF IRAN[Internet]. 2002;13(2):107-112. Available from: https://sid.ir/paper/529081/en

IEEE: Copy

S.M. TABATABAEI YAZDI, S. KHALEGHPARAST, and H.R. MONSEF, “PURIFICATION AND SOME PARTIAL CHARACTERIZATION OF PEROXIDASE ISOENZYME FROM BRASSICA OLERACEA CAPITATA L.,” JOURNAL OF SCIENCES ISLAMIC REPUBLIC OF IRAN, vol. 13, no. 2, pp. 107–112, 2002, [Online]. Available: https://sid.ir/paper/529081/en

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